We narrowed to 1,213 results for: Aspa

1. pCu-Atg17(416) (I325D)

   Plasmid

   #121407

   Purpose

   Expressing Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-ATG17 ORF-CYC1 terminator
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Isoleucine 325 to Aspartic acid

   Promoter

   CUP1 promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. N229A Lo-MetQ

   Plasmid

   #118268

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Insert

   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x His Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala)

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a - Ec-ClpX D144L

   Plasmid

   #111514

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Aspartatic acid 144 to Leucine

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 human A3C.D99E

   Plasmid

   #105049

   Purpose

   Overexpression of APOBEC3C D99E mutant in mammalian cell lines

   Depositor

   Carsten Münk

   Article

   Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

   Insert

   APOBEC3C (A3C) (APOBEC3C Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Aspartate 99 to glutamate

   Promoter

   CMV

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK524

   Plasmid

   #71580

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes asparagine-347 to alanine

   Promoter

   T7

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK517

   Plasmid

   #71579

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-435 to aspartate

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSP64-hERG1a D540K

   Plasmid

   #53057

   Purpose

   Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Aspartate 540 to Lysine

   Promoter

   SP6

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3 T7 BimL(S44A,DE)

   Plasmid

   #25054

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL (S44A,DE) (BCL2L11 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Changed serine 44 to alanine changed leucine 92 …

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3 T7 BimL(T56A,DE)

   Plasmid

   #25055

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL (T56A,DE) (BCL2L11 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Changed threonine 56 to alanine changed leucine …

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3 T7 BimL(S58A,DE)

    Plasmid

    #25056

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S58A,DE) (BCL2L11 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed serine 58 to alanine changed leucine 92 …

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3 Flag Bmf (S74A,S77A)

    Plasmid

    #24262

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bmf (S87A,S90A) (BMF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 74 to Alanine changed Serine 77 t…

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

12. TPC2-D276K-G-GECO1.2

    Plasmid

    #207142

    Purpose

    Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Changed Aspartate 276 to Lysine

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DsRed-rab11 DN

    Plasmid

    #12680

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-rab11 DN

    Plasmid

    #12678

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PHB2 S39D-mCherry

    Plasmid

    #232787

    Purpose

    Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    PHB2 (PHB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 39 to Aspartate

    Promoter

    CMV

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcmv3-IRG1-N152S-HA

    Plasmid

    #198182

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) N152S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The asparagine in IRG1 at position 152 mutates to…

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs-IP- SCAT3.2

    Plasmid

    #172497

    Purpose

    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Amcyan
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-mEGFP-CaMKIIa (T286D)

    Plasmid

    #127391

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge