We narrowed to 8,850 results for: NAP

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pLV-PGK-SNAP-MD-SOX2

Plasmid

#115687

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MD-SOX2

Use

Lentiviral

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

Plasmid

#100049

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation

Depositor

Karl Deisseroth

Insert

hChR2(E123T/H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

humanized, E123T/H134R

Promoter

hSynap

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

Plasmid

#106184

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

hSynapsin

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-7 [N275/14R]

Plasmid

#177526

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-7 (Mouse). Derived from hybridoma N275/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-7 (Mus musculus) recombinant mouse monoclonal antibody (Syt7 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for H3f3b

Plasmid

#186933

Purpose

Genomic targeting of SNAP tag at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for H3f3b (H3f3b Mouse)

Tags

SNAP tag

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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mCit-Snap25b D179K A199R

Plasmid

#53259

Purpose

SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Mutation

D179K and A199R

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only
Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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ZM (pyrFEKO-HYG TetR-T7RNAP)

Plasmid

#31455

Depositor

George Cross

Insert

Use

Floxed targeting vector for trypanosome

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
KIF5A-SNAPf-HisTag-FLAG

Plasmid

#200791

Purpose

Mammalian expression of KIF5A-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

SNAPf, HisTag, FLAG

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB-SNAP

Plasmid

#166146

Purpose

Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10 and SNAP

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10 and SNAP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synapsin-3 [L125/76R]

Plasmid

#206608

Purpose

Mammalian Expression Plasmid of anti-Synapsin-3 (Human). Derived from hybridoma L125/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synapsin-3 (Homo sapiens) recombinant Mouse monoclonal antibody (SYN3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hSynapsin-Nluc-EPAC-VV

Plasmid

#204846

Purpose

Produce lentivirus encoding Nluc-EPAC sensor in neuronal cell type

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

Nluc-EPAC sensor

Use

Lentiviral

Available Since

Aug. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-9 [N276A/15R]

Plasmid

#188223

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-9 (Mouse). Derived from hybridoma N276A/15

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-9 (Mus musculus) recombinant mouse monoclonal antibody (Syt9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-6 [N270/47R]

Plasmid

#188216

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-6 (Mouse). Derived from hybridoma N270/47

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-6 (Mus musculus) recombinant mouse monoclonal antibody (Syt6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2-Synapsin1a

Plasmid

#191567

Purpose

Expresses an shRNA and mTagBFP2-synapsin1a

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for Hist1h3g

Plasmid

#186932

Purpose

Genomic targeting of SNAP tag at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for Hist1h3g (H3c8 Mouse)

Tags

SNAP

Expression

Mammalian

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MD-OCT4

Plasmid

#115691

Purpose

Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Article

Friman et al Elife. 2019 Dec 3;8. pii: 50087. doi: 10

Insert

SNAP-MD-OCT4

Use

Lentiviral

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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