We narrowed to 5,043 results for: UBC

1. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

   Plasmid

   #225939

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   STIM1 (STIM1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

   Plasmid

   #225940

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   STIM2 (STIM2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

   Plasmid

   #225944

   Purpose

   Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   ATL1 (ATL1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #218637

   Purpose

   Proximity biotinylation control of OSBP(PH domain)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   OSBP (OSBP Human)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   PH domain only

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

   Plasmid

   #216477

   Purpose

   lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

   Plasmid

   #55221

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29, 117-160 and 412-496 Bactrocera parkin

   Plasmid

   #128384

   Purpose

   expresses protein UbcH7 (C86K) fused to Bactrocera parkin with residues 1-29, 117-160 and 412-496 deleted

   Depositor

   Kalle Gehring

   Article

   Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-

   Insert

   UbcH7 (UBE2L3 Human)
   

   Use

   Tags

   Bactrocera dorsalis parkin deleted residues 1 to …

   Expression

   Bacterial

   Mutation

   mutation C86K in UbcH7 gene

   Promoter

   lac promotor

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

    Plasmid

    #194278

    Purpose

    Expresses gRNA and dSaCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized dSaCas9 KRAB T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hUbC

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

    Plasmid

    #201186

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 1 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

    Plasmid

    #184556

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

    Plasmid

    #184557

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

    Plasmid

    #215012

    Purpose

    Express a ERphagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    KDEL
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

    Plasmid

    #201187

    Purpose

    Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    PTPN2, transcript variant 2 (PTPN2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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