We narrowed to 12,455 results for: cas9 expression plasmid

1. p1195-pssAAV.U1a.hNme2Cas9

   Plasmid

   #129534

   Purpose

   AAV vector expressing Nme2Cas9

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Insert

   human codon-optimized Nme2Cas9
   

   Use

   AAV

   Tags

   2xNLS and NLS-3xHA-NLS

   Expression

   Mutation

   Promoter

   U1a

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

   Plasmid

   #214814

   Purpose

   A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

   Depositor

   Yannick Doyon

   Insert

   St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

   Plasmid

   #214814

   Purpose

   A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

   Depositor

   Yannick Doyon

   Insert

   St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_dCas9-2xAM_hIRF-1

   Plasmid

   #92221

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA targeting human IRF-1 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_dCas9-2xAM_hIRF-1

   Plasmid

   #92221

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA targeting human IRF-1 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

    Plasmid

    #169097

    Purpose

    Cas9 and Citrine expression in transfected cells

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    Cas9-2A-Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

    Plasmid

    #169097

    Purpose

    Cas9 and Citrine expression in transfected cells

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    Cas9-2A-Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9(BB)-2A-Puro-SMCR8

    Plasmid

    #83440

    Purpose

    Expresses Cas9 and a gRNA targeting SMCR8

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human SMCR8 (SMCR8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCas9(BB)-2A-Puro-SMCR8

    Plasmid

    #83440

    Purpose

    Expresses Cas9 and a gRNA targeting SMCR8

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human SMCR8 (SMCR8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lv EF1a dCas9-HA 2A Blast

    Plasmid

    #102812

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    dCas9-HA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A (dCas9)

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lv EF1a dCas9-HA 2A Blast

    Plasmid

    #102812

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    dCas9-HA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A (dCas9)

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFREE2-SpCas9-sgRNA

    Plasmid

    #179582

    Purpose

    Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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