We narrowed to 1,086 results for: gibson assembly

1. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKG1334_pHarbor-PiggyBac_backbone

   Plasmid

   #239705

   Purpose

   PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAWP78-PVCpnf_pvc12-HiBiT

   Plasmid

   #198308

   Purpose

   Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc12-HiBiT
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcCAST-sgRNA_entry (CJT83)

   Plasmid

   #181785

   Purpose

   Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Expression

   Bacterial

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMulti-sgRNA-LacZ-DsRed

   Plasmid

   #99914

   Purpose

   Receptor plasmid for the assembly of multiple sgRNAs with DsRed coexpression

   Depositor

   Yujie Sun

   Article

   Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShoCAST-sgRNA_entry (BO1)

   Plasmid

   #181786

   Purpose

   Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
   

   Expression

   Bacterial

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pKG1179_pPV1-EFS

   Plasmid

   #239690

   Purpose

   BsaI Golden Gate assembly plasmid encoding the EFS promoter

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   EFS promoter
   

   Use

   Synthetic Biology

   Available Since

   Oct. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNC-Y

   Plasmid

   #238998

   Purpose

   Node C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY

   Depositor

   Javier Santos-Moreno

   Article

   Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

   Insert

   sgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHW621

    Plasmid

    #229867

    Purpose

    Entry vector with cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR in slot 5 (for SapTrap assembly)

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
    

    Expression

    Worm

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKG0586_pPV2-mRuby2

    Plasmid

    #239695

    Purpose

    BsaI Golden Gate assembly plasmid encoding the mRuby2 CDS

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG2039_pPV1-CAG

    Plasmid

    #239686

    Purpose

    BsaI Golden Gate assembly plasmid encoding the CAG promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    CAG promoter
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. MZB164

    Plasmid

    #217842

    Purpose

    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    HaloTag
    

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHW1143

    Plasmid

    #229866

    Purpose

    Entry vector with FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA in slot 4 (for SapTrap assembly)

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA
    

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CcCDP_p15A

    Plasmid

    #179272

    Purpose

    Expresses CcCDP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Insert

    cellodextrin phosphorylase
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PMXS-GOT1

    Plasmid

    #72872

    Purpose

    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    GOT1 (GOT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    sgRNA resistant human GOT1, 7 silent mutations c1…

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pUCC001

    Plasmid

    #124451

    Purpose

    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HH-BsaI
    

    Expression

    Yeast

    Mutation

    gRNA expression cassette from pUDP002 modified to…

    Promoter

    TDH3 promoter from S.cerevisiae (already present …

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet3-H_I3

    Plasmid

    #239343

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Tags

    mScarlet3-H (a.k.a. mYongHong)

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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