We narrowed to 1,096 results for: gibson assembly
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Plasmid#233924PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutationDepositorInsertCHARMS-1xA
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-3x
Plasmid#233925PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifsDepositorInsertCHARMS-3x
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-3xA
Plasmid#233926PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutationDepositorInsertCHARMS-3xA
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-5x
Plasmid#233927PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifsDepositorInsertCHARMS-5x
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-5xA
Plasmid#233928PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutationDepositorInsertCHARMS-5xA
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-S34Y
Plasmid#233929PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formationDepositorInsertCHARMS-S34Y
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-11xTA
Plasmid#233932PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domainDepositorInsertCHARMS-11xTA
UseLentiviralTagsmEGFPExpressionMammalianPromoterSV40Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen
Plasmid#196974PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-sgGOT1
Plasmid#72874PurposeThe lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).DepositorInsertsgGOT1
UseLentiviralAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKG1334_pHarbor-PiggyBac_backbone
Plasmid#239705PurposePaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integrationDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKG1179_pPV1-EFS
Plasmid#239690PurposeBsaI Golden Gate assembly plasmid encoding the EFS promoterDepositorInsertEFS promoter
UseSynthetic BiologyAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC-Y
Plasmid#238998PurposeNode C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZYDepositorInsertsgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
UseSynthetic BiologyExpressionBacterialMutationWTAvailable SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW621
Plasmid#229867PurposeEntry vector with cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR in slot 5 (for SapTrap assembly)DepositorInsertcGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
ExpressionWormAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0586_pPV2-mRuby2
Plasmid#239695PurposeBsaI Golden Gate assembly plasmid encoding the mRuby2 CDSDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2039_pPV1-CAG
Plasmid#239686PurposeBsaI Golden Gate assembly plasmid encoding the CAG promoterDepositorInsertCAG promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB164
Plasmid#217842Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expressionDepositorInsertHaloTag
Tags10x His, HaloTag, and SUMO (SMT3)ExpressionBacterialPromoterT7 PromoterAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only