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Addgene

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We narrowed to 1,525 results for: mbp

Showing: 961 - 980 of 1525 results

  1. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

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  2. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

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  3. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Use
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

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Showing: 961 - 980 of 1525 results