We narrowed to 2,137 results for: pam

1. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

   Plasmid

   #208697

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-GRAB-rDA2mut

   Plasmid

   #208705

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_rDA2mut in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) control sensor GRAB_rDA2mut
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

   Plasmid

   #208695

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-GRAB-HaloDA1.0

   Plasmid

   #234408

   Purpose

   Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

   Depositor

   Yulong Li

   Article

   Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

   Insert

   GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

   Plasmid

   #234410

   Purpose

   Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

   Insert

   GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDisplay-GRAB-HaloDAmut-IRES-EGFP-CAAX

   Plasmid

   #234411

   Purpose

   Expresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

   Insert

   GPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

   Plasmid

   #209199

   Purpose

   Mutagenesis of Kcnma1

   Depositor

   Larry Zweifel

   Article

   Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

   Insert

   Kcnma1 (Kcnma1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. peSCBE3-NG

   Plasmid

   #218163

   Purpose

   This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cbh_v5 AAV-saCBE_KKH C-terminal

    Plasmid

    #137184

    Purpose

    AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE_KKH C-terminal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E782K;N968K;R1015H conferring recognition of NNNR…

    Promoter

    Cbh

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXS-IRES-BLAST flag-CAD

    Plasmid

    #169818

    Purpose

    Expresses C-terminal flag-tagged CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCCC -> AGTC silent mutations at nt527-530 eli…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXS-IRES-BLAST flag-CAD S1859A

    Plasmid

    #169821

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859A; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. WT1 KI gRNA1

    Plasmid

    #92312

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. WT1 KI gRNA2

    Plasmid

    #92313

    Purpose

    CRISPR-GFP-gRNA for cutting WT1

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    CRISPR GFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-BLAST Halo-flag-CAD

    Plasmid

    #188117

    Purpose

    Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag and Halo tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. peSCBE3-NG-HF1

    Plasmid

    #218164

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXS-IRES-BLAST flag-CAD E1368A

    Plasmid

    #188141

    Purpose

    Expresses C-terminal flag-tagged human CAD E1368A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD S1396A

    Plasmid

    #188126

    Purpose

    Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge