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pYPQAT06

Plasmid

#223378

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO4-2(wt)

Plasmid

#191802

Purpose

Full length wt AtPCO4-2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO4-2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-FL

Plasmid

#216515

Purpose

Expresses MBP-tagged full length hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2 FL

Tags

6xHis tags-MBP

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Plasmid

#233446

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-PCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Plasmid

#233442

Purpose

PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Plasmid

#233444

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-MCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)

Plasmid

#233427

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

LIDARsensor(mcherry-BFP)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

Plasmid

#233413

Purpose

LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ054 (PB)SFFV-LIDARsensor (hygR)

Plasmid

#233419

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM

Plasmid

#233429

Purpose

Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-LIDARsensor(W)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP

Plasmid

#233408

Purpose

LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xPP7))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

Plasmid

#233415

Purpose

LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

Plasmid

#233439

Purpose

SFFV driven LIDAR reporter with 2 tandem substrate stem loops

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM044-3 CMV-TO-CCR7-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233395

Purpose

CCR7-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR7-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM105 CMV-TO-CX3CR1-(noV2)-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233397

Purpose

CX3CR1-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CX3CR1-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM068 CMV-TO-CX3CR1-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233398

Purpose

CX3CR1-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CX3CR1-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ113 CMV-TO-signal-hOXTR-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233399

Purpose

human oxytocin receptor-ADAR2dd(E488Q, T501A), no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

OXTR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ105 CMV-TO-signal-hOXTR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233400

Purpose

human oxytocin receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

OXTR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ142 CMV-TO-signal-B2AR-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233401

Purpose

_-2 adrenergic receptor-ADAR2dd(E488Q, T501A), no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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