We narrowed to 10,125 results for: Uty

1. pLKO_FLAG.NWS.C_NGFR

   Plasmid

   #158258

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG.NWS.C

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_HA.NWS.C_NGFR

   Plasmid

   #158261

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.NWS.C

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN40C.EFS.eGFP.ARID3A-3'UTR-WT

   Plasmid

   #169297

   Purpose

   Constitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   ARID3A-3'UTR-WT (ARID3A Human)
   

   Use

   Lentiviral

   Promoter

   EFS

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR

   Plasmid

   #128507

   Purpose

   Lentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-M-4F2

   Plasmid

   #73431

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 4F2 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-M-1B6

   Plasmid

   #73430

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1B6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pdCas9-M-3H5

   Plasmid

   #73429

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 3H5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pdCas9-M-1D4

   Plasmid

   #73433

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1D4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pdCas9-M-1E4

   Plasmid

   #73436

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1E4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pdCas9-M-5F5

    Plasmid

    #73432

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 5F5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV Hygro DHFR.dn-cHSF1

    Plasmid

    #134731

    Purpose

    Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    DHFR.dn-cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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12. CCR5-SZ218a QTY

    Plasmid

    #162444

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Availability

    Academic Institutions and Nonprofits only

    

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13. CCR5-SZ190b QTY

    Plasmid

    #162445

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2/shCol1a1/GFP4_Seq1.3

    Plasmid

    #206136

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2/shCol1a1/Scarlet_Seq1.3

    Plasmid

    #206137

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MG1655 Z1 malE clpA

    Bacterial Strain

    #65918

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

17. MG1655 Z1 malE aat

    Bacterial Strain

    #65917

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

18. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    K3753R

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-hCCND1-HA

    Plasmid

    #174154

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX313-TP53-WT

    Plasmid

    #118014

    Purpose

    Constitutive expression of wild-type human Tumor Protein p53 (TP53)

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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