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pET-Cre-LPETG-6xHis

Plasmid

#237224

Purpose

Expresses LPETG-tagged Cre recombinase in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Cre-LPETG (cre Synthetic)

Tags

6xHis

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMH-SpAsp1

Plasmid

#238315

Purpose

SpAsp1 protein expression in bacteria

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

asp1 (asp1 Fission Yeast)

Tags

His7-Tag, Strep-tag II, SUMO protein

Expression

Bacterial

Mutation

SpAsp1

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP2-VP2-454TAG

Plasmid

#240060

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP2

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP2
VP2-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-454TAG

Plasmid

#240059

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP1

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP1
VP1-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 HA PTPL1

Plasmid

#237335

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

PTPL1 (PTPN13 Human)

Tags

HA

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG p85beta

Plasmid

#237336

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

p85beta (PIK3R2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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PC2328

Plasmid

#232089

Purpose

Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.

Depositor

Pranam Chatterjee

Article

Hong et al Nat Commun. 2025 Apr 15;16(1):3555. doi:

Insert

duAb with cloning Esp3I restriction site

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK121 [Misc_CEN6-ARS4-HIS3_PartType11]

Plasmid

#229358

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Misc_CEN6-ARS4-HIS3_PartType11

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

CEN6-ARS4-HIS3

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (21 kDa)

Plasmid

#231634

Purpose

Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Expression

Mammalian

Mutation

Deletion of C-terminus (aa181-225)

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MZB215

Plasmid

#221767

Purpose

Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf6 (ARF6 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q67L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB218

Plasmid

#221769

Purpose

Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf4 (ARF4 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB219

Plasmid

#221770

Purpose

Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf5 (ARF5 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-7

Plasmid

#228989

Purpose

For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-38

Plasmid

#228979

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-38

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-44

Plasmid

#228981

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-44

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13CDS

Plasmid

#192853

Purpose

Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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