We narrowed to 31,214 results for: PLE;
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Plasmid#213282PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-ABE8e-V106W
Plasmid#193653PurposeExpression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-TadA*8e-V106W, EGFP
ExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, TadA*8e-V106WPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL40C_PGKintron_Cas9_Green
Plasmid#134966PurposeLentiviral vector coding Cas9 and mNeonGreenDepositorInsertCas9-P2A-mNeonGreen
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterhPGK promoter with beta-globin intronAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP
Plasmid#55645PurposeCre-on/Flp-on ChR2-EYFP under the Synapsin promoterDepositorHas ServiceAAV Retrograde, AAV5, AAV8, and AAV9InsertChR2(H134R)-EYFP
UseAAV; Cre on/flp on chr2-eyfpTagsEYFPExpressionMammalianPromoterhSynAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Foff hChR2(H134R)-EYFP
Plasmid#55646PurposeCre-on/Flp-off ChR2-EYFP under the Synapsin promoterDepositorInsertChR2(H134R)-EYFP
UseAAV; Cre on/flp off chr2-eyfpTagsEYFPExpressionMammalianPromoterhSynAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC3 CTF-FLAG
Plasmid#217686PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR3 (CELSR3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2516Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP
Plasmid#55648PurposeCre-off/Flp-on ChR2-EYFP under the Synapsin promoterDepositorInsertChR2(H134R)-EYFP
UseAAV; Cre off/flp on chr2-eyfpTagsEYFPExpressionMammalianPromoterhSynAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only