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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 128,596 results for: CIL

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Showing: 9901 - 9920 of 128596 results

  3. pX552-GFP

    Plasmid
    #107023
    Purpose
    pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.
    Depositor
    Alex Hewitt
    Insert
    GFP
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107023-plasmid-map-sequence-id-208521

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  8. pcDNA3.1_crGE

    Plasmid
    #172933
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.
    Depositor
    Arnold Boersma
    Publication
    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CrowdingSensorGE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172933-plasmid-map-sequence-id-339481

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  10. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47912-plasmid-map-sequence-id-68534

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  15. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

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  18. pMB66

    Plasmid
    #47946
    Purpose
    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP
    Expression
    Worm
    Promoter
    hsp-16.48
    Available since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    47946-plasmid-map-sequence-id-68806

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Showing: 9901 - 9920 of 128596 results