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pINDUCER10‐miR CNOT1

Plasmid

#251030

Purpose

allows tetracycline-inducible expression of an shRNA targeting human CNOT1

Depositor

Lienhard Schmitz

Publication

Rodriguez-Gil et al Sci Rep. 2017 Jun 14;7(1):3547. doi: 10.

Insert

shRNA against CNOT1 (CNOT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

tet-inducible

Available since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_TRNAU1AP (pAVA3291)

Plasmid

#254883

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting TRNAU1AP

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Two sgRNAs targeting TRNAU1AP

Use

CRISPR and Lentiviral

Promoter

U6/7SK

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_PRPF39 (pAVA3245)

Plasmid

#254885

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting PRPF39

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Two sgRNAs targeting PRPF39

Use

CRISPR and Lentiviral

Promoter

U6/7SK

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B (pAVA2834)

Plasmid

#254887

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin without sgRNA used as backbone

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD YuzuFP

Plasmid

#240149

Purpose

Expresses YuzuFP in E. coli using arabinose induction

Depositor

Dafydd Jones

Publication

Ahmed et al Commun Chem. 2025 Jun 5;8(1):174. doi: 1

Insert

YuzuFP

Tags

6xHis tag

Expression

Bacterial

Mutation

H148S

Promoter

Arabinose

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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wc-2[mAp]_hph

Plasmid

#245516

Purpose

Endogenous C-terminal tag the circadian clock positive arm component WC-2 with mApple.

Depositor

Jay C Dunlap

Publication

Wang et al J Cell Biol. 2026 Jun 1;225(6):e20250904

Insert

wc-2

Use

Neurospora expression

Tags

mApple

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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csr-1_frq[mCherry_V5H6]

Plasmid

#245517

Purpose

Epitopically express mCherry-tagged FRQ driven by frq endogenous promoter at csr-1 locus.

Depositor

Jay C Dunlap

Publication

Wang et al J Cell Biol. 2026 Jun 1;225(6):e20250904

Insert

frq

Use

Neurospora expression

Tags

V5H6 and mCherry

Promoter

frq promoter

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ180:[pA-BD9-S-BD5-S-(5'D)-EC-Ex1]-CMVp-[(G3p)RC-mK2-Ex1-miRS2-mK2-Ex2-miRS1-BSs-miRS3-BSs-pA]

Plasmid

#251311

Purpose

Expression of mKate2 Exon 1 and 2 and ECFP-Ex1 for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mKate2 Exon 1 and 2 and EC-Ex1

Expression

Mammalian

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR80:H2A1p-BD6-AS (50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251289

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR81:SSX1p-mK2-Ex1-(5'D)-ID-BD5-S(50bp, 50%GC)-pA

Plasmid

#251290

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR83:H2A1p-BD5-AS(50bp, 50%GC)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251291

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR84:SSX1p-mK2-Ex1-(5'D)-ID-BD7-S (30bp, 36%GC)-pA

Plasmid

#251292

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR85:H2A1p-BD7-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251293

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR86:SSX1p-mK2-Ex1-(5'D)-ID-BD8-S (30bp, 36%GC)-pA

Plasmid

#251294

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR87:H2A1p-BD8-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251295

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR88:SSX1p-mK2-Ex1-(5'D)-ID-BD9-S (30bp, 36%GC)-pA

Plasmid

#251296

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR89:H2A1p-BD9-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251297

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR90:SSX1p-mK2-Ex1-(5'D)-ID-BD10-S (30bp, 30%GC)-pA

Plasmid

#251298

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR91:H2A1p-BD10-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251299

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR19:SSX1p-mK2-Ex1-(5'D)-ID-BD3-S-(30bp 50%GC)-(linker1)-pA

Plasmid

#251277

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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