We narrowed to 374 results for: petD;

1. pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT)

   Plasmid

   #223733

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   NIX (BNIP3L) soluble part (BNIP3L Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   aa 1-182 only

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT)

   Plasmid

   #223734

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   FUNDC1 soluble part (FUNDC1 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   aa 1-50 only

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETDUET-GST AO7RE Y165A

   Plasmid

   #139428

   Purpose

   GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed tyrosine 165 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETDUET-GST AO7RE P199A

   Plasmid

   #139427

   Purpose

   GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed proline 199 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETDUET-GST AO7RE I137A

   Plasmid

   #139319

   Purpose

   GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed isoleucine 137 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet RagA(R37P)-RagC

   Plasmid

   #99646

   Purpose

   Bacterial expression of RagA(R37P)-RagC

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   R37P

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETDuet RagA-RagC(K84T))

   Plasmid

   #99648

   Purpose

   Bacterial expression of RagA-RagC(K84T)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   K84T

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   Lambda-PPase
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. [pSK122] pETDuet GST-p18(delta5)

   Plasmid

   #136144

   Purpose

   bacterial expression of GST-p18

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

   Insert

   GST-p18
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet-1 Hepatitis C Virus NS3/NS4A

    Plasmid

    #61696

    Purpose

    inducible dual expression of NS3 protease along with its cofactor NS4A

    Depositor

    Michael Johnson

    Article

    Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

    Inserts

    NS4A
    NS3
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

    Plasmid

    #61939

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

    Plasmid

    #61938

    Purpose

    Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF2) (UCHL5 Human)
    hNFRKB (39-156) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 2-38 dele…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

    Plasmid

    #61943

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Residues 2-39 deleted resulting in construct star…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)

    Plasmid

    #223736

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)

    Plasmid

    #223737

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    NIX (BNIP3L) soluble part (BNIP3L Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

    Plasmid

    #223745

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    BCL2L13 soluble part (BCL2L13 Human)
    

    Use

    Tags

    EGFP-TEV-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDUET-GST AO7RE ∆238-245

    Plasmid

    #139318

    Purpose

    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDuet1-LanCL2-H264A-C-Ter-His

    Plasmid

    #154187

    Purpose

    To express LanCL2-H264A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His264->Ala264

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge