1. p426 72Q GPD

   Plasmid

   #1183

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

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1. MtMscL delta49

   Plasmid

   #118342

   Purpose

   M. tuberculosis MscL (delta49) in pET19b

   Depositor

   Douglas Rees

   Article

   Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

   Insert

   MscL (mscL Mycobacterium tuberculosis)
   

   Use

   Tags

   6x His Tag and Thrombin Cleavage site

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 102 to Stop Codon

   Promoter

   T7 Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-Craf SSAA

   Plasmid

   #118336

   Purpose

   Expression of human Craf

   Depositor

   Philip Stork

   Article

   Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

   Insert

   Craf SSAA (RAF1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   SSAA

   Promoter

   CMV

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Gal4-HSF4b K294R

   Plasmid

   #118359

   Purpose

   This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K294R

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hHSF4b K294R

   Plasmid

   #118357

   Purpose

   This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K294R

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Flag-Fos S362DS374A

   Plasmid

   #118305

   Purpose

   Expression of mous C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S362DS374A (Fos Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S362DS374A

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-Fos S362AS374D

   Plasmid

   #118304

   Purpose

   Expression of mouse C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S362AS374D (Fos Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S362AS374D

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-Fos S362D

   Plasmid

   #118303

   Purpose

   Expression of mouse C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S362D (Fos Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S362D

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-KSR R615H

   Plasmid

   #118332

   Purpose

   Expression of mouse KSR

   Depositor

   Philip Stork

   Article

   Takahashi et al J Biol Chem. 2017 Jan 27;292(4):1449-146

   Insert

   KSR R615H (Ksr1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R615H

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Gal4-HSF4b WT

   Plasmid

   #118358

   Purpose

   This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hHSF1 S307A

    Plasmid

    #118364

    Purpose

    This plasmid expresses human HSF1 S307A mutant.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR-NLS-actin-R62D

    Plasmid

    #118381

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    NLS and R62D

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-actin

    Plasmid

    #118378

    Purpose

    Gateway compatible donor vector with WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUPER-scramble

    Plasmid

    #118349

    Purpose

    This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    scrambled shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-NLS-actin

    Plasmid

    #118380

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    NLS

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hHSF1 RNAi resistant

    Plasmid

    #118347

    Purpose

    This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    RNAi resistance mutations (does not change amino …

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUPER-hHSF1 shRNA

    Plasmid

    #118345

    Purpose

    This plasmid expresses shRNA against hHSF1.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 shRNA (HSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-X1-hygro-mCherry-RAMP4

    Plasmid

    #118391

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hSUMO1-HIS

    Plasmid

    #118361

    Purpose

    This plasmid expresses the full length human SUMO1.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hSUMO1 (SUMO1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge