We narrowed to 314,746 results for: addgene

1. KSHV Tiling Library

   Pooled Library

   #180272

   Purpose

   sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.

   Depositor

   Britt Glaunsinger

   Article

   Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.

   Expression

   Mammalian

   Species

   Kaposi’s sarcoma-associated herpes virus

   Use

   Lentiviral

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

2. mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

   Pooled Library

   #197349

   Purpose

   To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

   Depositor

   Jonathan Weissman, Rebecca Voorhees

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Species

   Homo sapiens

   Use

   Lentiviral

   Available since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

3. Inzolia library in pRDA_550

   Pooled Library

   #209552

   Purpose

   The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Use

   CRISPR and Lentiviral

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

4. Inzolia library in pRDA_052

   Pooled Library

   #209551

   Purpose

   The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Use

   CRISPR and Lentiviral

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

5. DNA repair library with 1,573 sgRNAs

   Pooled Library

   #177663

   Purpose

   CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Species

   Homo sapiens

   Use

   CRISPR

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

6. Humagne Set C

   Pooled Library

   #172650

   Purpose

   Set C contains best-ranked guides 1 and 4.

   Depositor

   John Doench, David Root

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Expression

   Mammalian

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

7. Humagne Set D

   Pooled Library

   #172651

   Purpose

   Set D contains best-ranked guides 2 and 3.

   Depositor

   John Doench, David Root

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Expression

   Mammalian

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

8. Lourido Toxoplasma CRISPR Library V1

   Pooled Library

   #80636

   Purpose

   Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. EFS-mTSG

   Pooled Library

   #113584

   Purpose

   AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

   Depositor

   Sidi Chen

   Article

   Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

   Expression

   Mammalian

   Use

   AAV, CRISPR, Lentiviral, and Luciferase

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

10. mTORC1 Focused library

    Pooled Library

    #164084

    Purpose

    Lentiviral CRISPR library targets human mTORC1 signaling pathway

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

11. Oxford Drosophila melanogaster Genome-wide CRISPR Library

    Pooled Library

    #64750

    Purpose

    A genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).

    Depositor

    Ji-Long Liu

    Article

    Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-

    Expression

    Insect

    Species

    Drosophila melanogaster, Streptococcus pyogenes, and Synthetic

    Use

    CRISPR

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

12. DNA repair library with 336 sgRNAs

    Pooled Library

    #177664

    Purpose

    CRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processes

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

13. mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

    Pooled Library

    #197348

    Purpose

    To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

    Depositor

    Jonathan Weissman, Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

14. RNA-binding protein pooled CRISPR knockout library

    Pooled Library

    #141438

    Purpose

    CRISPR knockout library targets RBPs with 10 sgRNAs/gene

    Depositor

    Eugene Yeo

    Article

    Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

15. The Human Druggable CRISPR library

    Pooled Library

    #200013

    Purpose

    The library contains ~10,000 sgRNAs targeting 1,980 human genes (5 sgRNAs per gene and 100 nontargeting control sgRNAs).

    Depositor

    Lianxin Liu

    Article

    Zheng et al Adv Sci (Weinh). 2023 Aug;10(22):e220679

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

16. Human SLC overexpression library

    Pooled Library

    #213694

    Purpose

    Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

17. Perturb-seq GBC library

    Pooled Library

    #85968

    Purpose

    Perturb-seq GBC library

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

18. TBG-mTSG

    Pooled Library

    #113585

    Purpose

    AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes in mouse liver

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Expression

    Mammalian

    Use

    AAV, CRISPR, Lentiviral, and Luciferase

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

19. Human sgRNA library Brunello in lentiGuide-Puro

    Pooled Library

    #73178

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Human sgRNA library Brunello in lentiCRISPRv2

    Pooled Library

    #73179

    Purpose

    Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only