We narrowed to 1,350 results for: biotin
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Plasmid#232584PurposeExpress HA-BirADepositorInsertBirA
TagsHAExpressionMammalianAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
Tags3xNLSExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)
Plasmid#29723DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mO…ExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICSL50014
Plasmid#50313DepositorInsertC terminal HBP tag (HA-PreScission-Biotin)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)
Plasmid#29724DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMLLKA-J72017
Plasmid#26008DepositorInsertN terminal avi tag with RBS
UseSynthetic Biology; 2ab assembly vectorAvailable SinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICSL50014
Plasmid#50313DepositorInsertC terminal HBP tag (HA-PreScission-Biotin)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLLKA-J72017
Plasmid#26008DepositorInsertN terminal avi tag with RBS
UseSynthetic Biology; 2ab assembly vectorAvailable SinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK24
Bacterial Strain#51950Purposebroad-host range natural conjugative plasmidDepositorBacterial ResistanceTetracyclineAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
C321.ΔA.exp
Bacterial Strain#49018PurposeThis is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 CDepositorBacterial ResistanceBleocin (Zeocin)SpeciesEscherichia coliAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-Biotin
Plasmid#206039PurposeHaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTagDepositorInsertTalin R3-IVVI (Tln1 Mouse)
Tags10 x His-AviTag and Halo-TagExpressionBacterialMutationThreonine 809 to Isoleucine, Threonine 833 to Val…PromoterT7Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C321.ΔA
Bacterial Strain#48998PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)DepositorBacterial ResistanceAmpicillinSpeciesEscherichia coliAvailable SinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTM-BPL
Plasmid#173766PurposeFluorescent labeling of the nuclear envelopeDepositorInsertPDGF receptor TM domain, Biotin protein ligase
TagsFLAG and Signal peptideExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-HA
Plasmid#188521PurposeEncodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianPromoterEF1a/RPBSAAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-NLS-BirA-2A-mCherry_Ras
Plasmid#80061PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_lbMicroID2-HA
Plasmid#188522PurposeEncodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cellsDepositorInsertlbMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianPromoterEF1a/RPBSAAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only