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We narrowed to 1,947 results for: biotin

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Showing: 81 - 100 of 1947 results

  7. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid
    #108570
    Purpose
    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi
    Insert
    dSpyCas9
    Use
    CRISPR and Lentiviral
    Tags
    APEX2, Destabilization domains, Flag, and mCherry…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    108570-plasmid-map-sequence-id-328916

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  9. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid
    #108570
    Purpose
    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi
    Insert
    dSpyCas9
    Use
    CRISPR and Lentiviral
    Tags
    APEX2, Destabilization domains, Flag, and mCherry…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    108570-plasmid-map-sequence-id-328916

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  13. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230058
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230058-plasmid-map-sequence-id-460119

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  14. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230059
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230059-plasmid-map-sequence-id-460118

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  15. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

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  16. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

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  18. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid
    #79885
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    79885-plasmid-map-sequence-id-150051

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  19. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  20. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80060
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80060-plasmid-map-sequence-id-152594

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Showing: 81 - 100 of 1947 results