We narrowed to 953 results for: dcas9, grna

1. PB_p300_core-dCas9-EGFP_hygro

   Plasmid

   #226431

   Purpose

   PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

   Depositor

   Alon Goren

   Inserts

   p300 core-dCas9-EGFP fusion protein
   rtTA3-IRES-Hygro
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   HA tag, P2A-EGFP, and p300 core

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible minimal CMV and UbC Promoter

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-M-G6

   Plasmid

   #73437

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor G6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdCas9-M-C4

   Plasmid

   #73427

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106354

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106352

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106355

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   MYOD1 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9 (GB1079)

   Plasmid

   #75399

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-M-3A2

    Plasmid

    #73435

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 3A2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdCas9-M-4A6

    Plasmid

    #73434

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4A6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdCas9-M-3F2

    Plasmid

    #73428

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor C4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdCas9-M-4F2

    Plasmid

    #73431

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4F2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdCas9-M-1B6

    Plasmid

    #73430

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1B6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pdCas9-M-3H5

    Plasmid

    #73429

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 3H5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pdCas9-M-1D4

    Plasmid

    #73433

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1D4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pdCas9-M-1E4

    Plasmid

    #73436

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1E4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdCas9-M-5F5

    Plasmid

    #73432

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 5F5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQdCas9-sggfp

    Plasmid

    #236184

    Purpose

    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Quorum sensing promoter

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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