Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,121 results for: gibson assembly

Clear keywords
Showing: 81 - 100 of 1121 results

  5. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    184225-plasmid-map-sequence-id-361604

    Enlarge

  7. EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190165
    Purpose
    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3N-VVDfast-mClover3-iLID (MAPRE3 Human, Synthetic)
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mammalian
    Mutation
    1-189 aa from EB3
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    190165-plasmid-map-sequence-id-374287

    Enlarge

  13. AdenoBuilder toolkit

    Plasmid kit
    #1000000176
    Purpose
    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.
    Depositor
    Fred Bunz
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Adenoviral, Mammalian Expression
    Cloning type
    Gibson
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. EB3N-VVDfast-Halo-iLID

    Plasmid
    #190166
    Purpose
    Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3N-VVDfast-Halo-iLID (MAPRE3 Human, Synthetic)
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mammalian
    Mutation
    1-189 aa from EB3
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    190166-plasmid-map-sequence-id-374286

    Enlarge

Showing: 81 - 100 of 1121 results