We narrowed to 747 results for: streptococcus -(crispr) -(cas9)

1. pHS1_10xHis_MBP_TEV_WT-Cas9

   Plasmid

   #244825

   Purpose

   Bacterial expression of SpyCas9 (WT) for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EC2_10_dCas9_VPR_HC_sgRNA

   Plasmid

   #163712

   Purpose

   Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHL-EF1a-SphcCas9(D10A)-iP-A

   Plasmid

   #60600

   Purpose

   Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   CRISPR Cas9 D10A
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Changed Asp 10 to Ala, Codon usage optimized for …

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pspCas9-3'UTR-ST2-com-vector

   Plasmid

   #136269

   Purpose

   Expresses SpCas9 and sgRNA Scaffold

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Insert

   SpCas9 and sgRNA scaffold
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

   Plasmid

   #101202

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   M1–N497, GGS, V713–D1368

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Plasmid

   #101233

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G582A/V583A/E584A/D585A/N588A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G582A, V583A, E584A, D585A and N588A

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc-sgRNA-Cas9

   Plasmid

   #49330

   Purpose

   Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid

    #101236

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K918A/V922A/R925A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K918A, V922A, R925A and Q926A

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid

    #101235

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    F491A, M495A, T496A, N497A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid

    #101234

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    T657A, G658A, W659A, R661A and Q926A

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

    Plasmid

    #101201

    Purpose

    Bacterial expression plasmid for SpCas9, REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C and S960C

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

    Plasmid

    #101200

    Purpose

    Bacterial expression plasmid for SpCas9, REC2 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C and D273C

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mesp-1916/-1>nls::Cas9::nls

    Plasmid

    #59988

    Purpose

    Mesp driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Mesp -1916/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB_Mut(D1399Y)_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226427

    Purpose

    PiggyBac transposon vector containing a dox inducible mutant p300 core-dCas9 fusion protein (p300 core mutation: D1399Y) and EGFP reporter. Hygromycin selection marker.

    Depositor

    Alon Goren

    Inserts

    Mutant p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, Mutant p300 core, and P2A-EGFP

    Expression

    Mammalian

    Mutation

    p300 core mutation (D1399Y)

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid

    #110624

    Purpose

    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    LP1B and hU6

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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