163,983 results

1. p6659 MSCV-IP N FlagHA HPV16E6

   Plasmid

   #44152

   Depositor

   Peter Howley

   Article

   White et al J Virol. 2012 Dec;86(24):13174-86. doi:

   Insert

   HPV16E6
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pHJ12: CadC-VHH-Caffeine (No linker)

   Plasmid

   #108244

   Purpose

   Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO1

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-45b-mCherry-NPM1

   Plasmid

   #194546

   Purpose

   Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   NPM1 (Npm1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-SaPE2

   Plasmid

   #174817

   Purpose

   Mammalian expression of SaCas9 prime editor 2

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB-UBC-GCAMP6f-PGK-neo

   Plasmid

   #160049

   Purpose

   expression of GCaMP6f in a piggybac plasmid

   Depositor

   Xiaojun Lian

   Insert

   GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF0056

   Plasmid

   #142529

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MAF (MAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MultiMate-Rainbow

   Plasmid

   #206252

   Purpose

   Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
   

   Use

   Recombinant baculovirus production (bac-to-bac)

   Tags

   iRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMG024 MBP-GSK3β-HA-His, GST_λPPase

   Plasmid

   #196182

   Purpose

   Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Use

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SPHK2 gRNA (BRDN0001148465)

   Plasmid

   #77093

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX304_zeo_mmIrf1

    Plasmid

    #160098

    Purpose

    Expresses murine Irf1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Irf1 (Irf1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge