We narrowed to 26,706 results for: vit;

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pGEX-4T2 Parkin RBR

Plasmid

#31255

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Parkin (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

RBR domain, residues 217-465

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pHIT182

Plasmid

#204511

Purpose

Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

Insert

csr-1a with D769A mutation (C. elegans) (csr-1 Nematode)

Tags

6xHis, MBP

Expression

Bacterial

Mutation

D769A

Promoter

T7lac

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GCN4 [IPI-C11L34] (Recombinant Antibody)

Antibody

#218104-rAb

Purpose

Anti-GCN4 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to HLENEVARLKK sequence.

Depositor

Institute for Protein Innovation (IPI)

Article

Zahnd et al J Biol Chem. 2004 Apr 30;279(18):18870-7

Recommended Applications

Western Blot

Reactivity

Budding yeast

Source Species

Rabbit

Isotype

IgG

Trial Size

Not available to purchase

Available Since

May 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits
FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37082

Purpose

Cre-dependent expression of hChR2(H134R) for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Promoter

EF1a

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcihygroflagsynhDAT

Plasmid

#19990

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

Dopamine transporter (SLC6A3 Human)

Tags

flag

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcihygrosynDAT

Plasmid

#19992

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

synDAT (SLC6A3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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ROZA

Plasmid

#64198

Purpose

"Reporter of Zap-70 Activity" Fluorescent reporter of ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

Plasmid

#68279

Purpose

SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Far1DS NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED73 pRS304 pRPS2-SteDS-NLS-mCitrine

Plasmid

#68278

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCitrine

Tags

mCitrine

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAcUW21-hovimeh1

Plasmid

#84211

Purpose

baculovirus transfer vector containing hovimeh1 coding sequence from Homalodisca vitripennis

Depositor

Bruce Hammock

Article

Kamita et al Arch Insect Biochem Physiol. 2013 Aug;83

Insert

meh1

Expression

Insect

Promoter

p10

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

Plasmid

#68276

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4E) mCherry

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

Plasmid

#68275

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4A) mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

Plasmid

#68280

Purpose

SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Mkk2DS NLS mCherry (MKK2 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T HHARI RBR

Plasmid

#31254

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Human Homologue of Ariadne (HHARI) (ARIH1 Human)

Tags

GST

Expression

Bacterial

Mutation

RBR domain residues 177-395

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

Plasmid

#182825

Purpose

Cre-dependent expression of H2B-APEX fusion

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

H2B.APEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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GBSGFP in pBS-KS

Plasmid

#121974

Purpose

Gli activity reporter

Depositor

Hiroshi Sasaki

Article

Sasaki et al Development. 1997 Apr . 124(7):1313-22.

Insert

GBSGFP (Gnb5 Mouse)

Use

Cloning

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-RELΔ424-490

Plasmid

#27267

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

Insert

REL (REL Human)

Expression

Mammalian

Mutation

Deletion of transactivation domain I (aa 425-490)

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00128

Plasmid

#34282

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3576 (LAG1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
scADGFP

Plasmid

#114434

Purpose

Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA

Depositor

Edward Perez-Reyes

Article

Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.

Insert

Green fluorescent protein

Use

AAV

Tags

ER export sequence FCYENEV

Expression

Mammalian

Promoter

Novel synthetic activity-dependent promoter

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

Plasmid

#51255

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Ala

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

Plasmid

#51257

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248,789,805,817,848,9…

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-TK-5UTR-BsmBI-Luciferase

Plasmid

#114670

Purpose

For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

Depositor

Lynne Postovit

Article

Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

Insert

LacZ

Use

Luciferase

Tags

for cloning 5' UTR-Luciferase fusions

Expression

Mammalian

Promoter

lac promoter

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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