-
-
pmirGLO-3'UTR-IL22
Plasmid#153070PurposeHuman IL-22 3'UTR region cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of IL-22 gene (IL22 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(214-598)_quadruple_mutant
Plasmid#135441PurposeExpresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationL535A, L539A, L546A, M549APromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)
Plasmid#110143PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseBinary vectorTagscogDsRedExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)
Plasmid#110144PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Fascin-Promoter (-210-0)
Plasmid#89826PurposeFascin promoter for luciferase assayDepositorInsertFascin1 (FSCN1 Human)
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-n
Plasmid#53065Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-n
Plasmid#53067Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-n
Plasmid#53071Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HLH Clock Pas 1-401 VenN L57E/L113E/W284A
Plasmid#47360PurposeClock fragment mutatation tagged with VenN used for BiFCDepositorInsertClock (Clock Mouse)
UseTagsVenus aa 1-155ExpressionMammalianMutationaa 1-401, L57E/L113E/W284APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC L95E
Plasmid#47365PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, L95EPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC L150E
Plasmid#47366PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, L150EPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC L95E/L150E/W427A
Plasmid#47372PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, L95E/L150E/W427APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC F423R
Plasmid#47368PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, F423RPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC W427A
Plasmid#47369PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, W427APromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC V435R
Plasmid#47370PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, V435RPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC F423R/V435R
Plasmid#47371PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, F423R/V435RPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-BHLHE40-IRES-ZsGreen1
Plasmid#153061PurposeLentiviral vector expressing human BHLHE40DepositorInsertBHLHE40 (BHLHE40 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-285
Plasmid#31060DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-285 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-Flag-FANCD2
Plasmid#134904Purposecodon optimized expression and purification of human FANCD2 in insect cells using baculovirusDepositorInsertFANCD2 (FANCD2 Human)
UseTagsFlagExpressionInsectMutationfully synthetic, codon optimized for insect expre…PromoterpolyhedronAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3
Plasmid#136959PurposeReporter vector encoding firefly luciferase and TurboRFP fluorescent proteinDepositorInsertFirefly luciferase and TurboRFP
UseLentiviralTagsV5 tagExpressionMutationPromoterCMVAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-1-NONO(53-312)
Plasmid#135442PurposeExpresses NONO 53-312 in the second site; for co-expression with His-tagged proteinDepositorInsertNon-POU domain-containing octamer-binding protein (NONO Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKD4-ChR2-YFP
Plasmid#178721PurposeAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)DepositorInsertChR2-YFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mScarlet-Giantin
Plasmid#196872PurposeExpresion of Giantin-fused to mScarlet. Used as Golgi markerDepositorInsertmScarlet-Giantin (GOLGB1 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA (pLP140)
Plasmid#168461PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM3
Plasmid#179957PurposeExpression vector for human IFITM3.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-2200
Plasmid#31062DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-2200 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Dual-StrepII-BARD1
Plasmid#137166PurposeExpresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell systemDepositorInsertBARD1 (BARD1 Human)
UseTagsStrepIIExpressionInsectMutationfully synthetic, codon optimized for insect expre…Promoterp10Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_A
Plasmid#74376PurposegRNA_A to knockout human AMPK alpha 2 using Cas9n.DepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_B
Plasmid#74377PurposegRNA_B to knockout human AMPK alpha 2 using Cas9nDepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-NEDD1-gTBD
Plasmid#196858PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD (NEDD1 Human)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-WT
Plasmid#74379PurposeLentiviral expression vector containing flag tagged WT MFF isoform 5DepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES
Plasmid#209851PurposeExpression of human codon-optimized light-driven sodium pump ErNaR in mammalian cellsDepositorInsertlight-driven sodium pump ErNaR
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA2 (pLP300)
Plasmid#168462PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC1GFP-Kir7.1
Plasmid#79118PurposeExpress N-terminal fused Kir7.1 protein in mammalian cellsDepositorInsertInwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBactin-EGFP-NEDD1-gTBD
Plasmid#196857PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertEGFP-N-gTBD (NEDD1 Human)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mCherry-p115(S240A)
Plasmid#181997PurposeMammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.DepositorInsertmCherry-p115(S240A) (ARHGEF1 Human)
UseTagsmCherryExpressionMammalianMutationSerine 240 changed to alanine in p115.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-257
Plasmid#31063DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-257 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-164
Plasmid#31066DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-164 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS5-ChR2-YFP
Plasmid#178725PurposeAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of ChR2-YFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInsertChR2-YFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only
