We narrowed to 1,529 results for: Flp

1. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

   Plasmid

   #52889

   Purpose

   "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Insect

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-FLEx(FRT)-TC

   Plasmid

   #67827

   Purpose

   Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   TVA-mCherry fusion
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMVTO-hIL2

   Plasmid

   #233387

   Purpose

   secreted human IL-2

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   IL-2 (IL2 Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO-EGFP-G3BP1-WT

   Plasmid

   #136003

   Purpose

   DOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

   Plasmid

   #166504

   Purpose

   Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

   Depositor

   Angelos Constantinou

   Article

   Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

   Insert

   None
   

   Tags

   TurboID and mCherry-CRY2

   Expression

   Mammalian

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. c3GIC9

   Plasmid

   #62191

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5B-FRT-AP-Smo-YFP-DEST

   Plasmid

   #49099

   Purpose

   Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Smo (Smo Mouse)
   

   Tags

   YFP and acceptor peptide (AP)

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDragon-empty

   Plasmid

   #155018

   Purpose

   Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

   Depositor

   Alberto Rosello-Diez

   Article

   Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

   Type

   Empty backbone

   Use

   Mouse Targeting

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FDIO-COX4-dAPEX2

   Plasmid

   #117178

   Purpose

   Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Mutation

    Enzymatically inactive Cre

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDragon-DTA

    Plasmid

    #155017

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activity

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    DTA
    

    Use

    Mouse Targeting

    Mutation

    tox176

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FDIO-LACTB-dAPEX2

    Plasmid

    #117181

    Purpose

    Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT344_pattB-pCA-GFP-pA-FRT5

    Plasmid

    #52550

    Purpose

    for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT TO-EGFP-G3BP1-S149A

    Plasmid

    #136004

    Purpose

    DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S149A

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FDIO-SYP-HRP

    Plasmid

    #117187

    Purpose

    Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW1997_pExpr-hU6-CRISPR-DECODER

    Plasmid

    #87549

    Purpose

    Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    gRNAs targeting NGN2, MIAT, ACTC1, TTN
    

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVBL0001

    Plasmid

    #242429

    Purpose

    Stable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.

    Depositor

    Vladislav Belyy

    Article

    Smith et al bioRxiv [Preprint]. 2025 Jul 21:2025.07.

    Insert

    IRE1alpha (ERN1 Human)
    

    Tags

    mCherry, CRY2clust

    Expression

    Mammalian

    Mutation

    Deletion of the lumenal domain

    Promoter

    CMV, tet-inducible

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. EW428 CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deImmLink-ZIK1KRAB

    Plasmid

    #236141

    Purpose

    Plasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-ZIK1KRAB
    

    Expression

    Mammalian

    Promoter

    CMV promoter with two TetR binding sites

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP

    Plasmid

    #236134

    Purpose

    Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    E1b minimal promoter with four ZNF35/ZNF250 bindi…

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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