We narrowed to 2,370 results for: fas

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pLenti-CaMKIIa-VChR1-EYFP-WPRE

Plasmid

#20954

Purpose

3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3b

Plasmid

#111056

Purpose

Expresses dLight1.3b in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3b

Tags

Flag tag

Expression

Mammalian

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCoofy1

Plasmid

#43974

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pPSmOrange2-Tubulin

Plasmid

#34963

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Tags

Tubulin

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 N-t

Plasmid

#89980

Purpose

Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

construct contains aa 1-182; deleted aa 183-361

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *VBM

Plasmid

#89976

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 282RKRR to 282HNHH

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-neo

Plasmid

#65231

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1 GBP (GBP-mCherry)

Plasmid

#162879

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

Plasmid

#83947

Purpose

gRNA cassette-carrying vector with natMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

gRNA targeting ADE2 gene

Expression

Yeast

Available Since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCoofy11

Plasmid

#44002

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10

Expression

Insect

Promoter

hr5

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6

Plasmid

#113887

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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PYL1_nTEVp

Plasmid

#119213

Purpose

Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nTEVp in sufion with PYL1

Tags

AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Calnexin-N-14

Plasmid

#231768

Purpose

Visualization of ER in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Calnexin-N-17 (CANX Human)

Tags

mGold2s

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDR125

Plasmid

#37150

Depositor

Dale Ramsden

Insert

lig4 (LIG4 Human)

Tags

H6

Expression

Insect

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

rrnB P1

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cas9-EGFP

Plasmid

#159126

Purpose

Expresses active Cas9 fused with EGFP for fluorescence microscopy

Depositor

Taekjip Ha

Article

Liu et al Science. 2020 Jun 12;368(6496):1265-1269

Insert

Cas9 fused to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MSCV LTR promoter

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2FA-H2B-mScarlet3

Plasmid

#224436

Purpose

Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

H2B-mScarlet3

Expression

Mammalian

Promoter

CMV & SP6

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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ABI_cTEVp

Plasmid

#119214

Purpose

Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cTEVp in fusion with ABI

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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