Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,628 results for: sgrna vector

Clear keywords
Showing: 981 - 1000 of 1628 results

  2. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

    Enlarge

  9. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

    Enlarge

  11. pDual_dsCas9_BFP

    Plasmid
    #214679
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214679-plasmid-map-sequence-id-428808

    Enlarge

  15. pV1081

    Plasmid
    #111425
    Purpose
    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    CaCas9/sgADE-NT2
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111425-plasmid-map-sequence-id-212268

    Enlarge

  17. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Available Since
    Oct. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68709-plasmid-map-sequence-id-125636

    Enlarge

  18. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid
    #140204
    Purpose
    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Step 1 of cloning c…
    Promoter
    Aspergillus fumigatus U3 (RNAPIII).
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    140204-plasmid-map-sequence-id-309884

    Enlarge

Showing: 981 - 1000 of 1628 results