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pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

Plasmid

#236368

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

Plasmid

#241144

Purpose

Expresses Anti-GCN4 scFv-StayGold, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-GCN4 scFv
tdMCP - CAAX

Tags

(n2)oxStayGold(c4) E147D and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET His6 Gamma crystallin TEV LIC cloning vector (1X)

Plasmid

#29661

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Gamma-crystallin, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 Gamma crystallin TEV LIC cloning vector (2X-T)

Plasmid

#29714

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Gamma crystallin-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

Plasmid

#199362

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

Plasmid

#99342

Purpose

Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

Depositor

Peter Wright

Article

Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

Inserts

STAT1 (710-750) (STAT1 Human)
CBP (1764-1855)

Tags

His6GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Plasmid

#159864

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-619 C-Tag Venus210[2] L10 Stat3(R609Q) mCherry

Plasmid

#130094

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBR25 CFAP36 [L438E, K442E]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238227

Purpose

CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E], and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [L438E, K442E]

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 CFAP36 [A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238228

Purpose

CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [A493E, K495A]

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Plasmid

#200793

Purpose

Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

1-914; Δ505-610

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRDAX-566 N-Tag GFP[1] L10 Stat3(R609Q) EBPF2

Plasmid

#130113

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-567 C-Tag GFP[1] L20 Stat3(R609Q) EBPF2

Plasmid

#130114

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-568 N-Tag GFP[1] L20 Stat3(R609Q) EBPF2

Plasmid

#130115

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-569 C-Tag GFP[1] L32 Stat3(R609Q) EBPF2

Plasmid

#130116

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-570 N-Tag GFP[1] L32 Stat3(R609Q) EBPF2

Plasmid

#130117

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-571 C-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130118

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130119

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-573 C-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130120

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-574 N-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130121

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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