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pre_IV_CS8_7569_v1

Plasmid

#126865

Purpose

7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Publication

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS8_7569_v1

Use

Synthetic Biology

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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ACV

Plasmid

#27791

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Amber-Cerulean-Venus

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-WW1-mutant

Plasmid

#17795

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-WW2-mutant

Plasmid

#17796

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 to Alanine Proline 261 to Alanine

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pSB1A3_GFP_dT34

Plasmid

#89669

Purpose

expression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT34

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLG327

Plasmid

#128797

Purpose

Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

Depositor

Christopher Voigt

Publication

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl(s)

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Fn

Plasmid

#108864

Purpose

FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

Depositor

Yiping Qi

Publication

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Type

Empty backbone

Use

CRISPR; Gateway compatible crrna entry clone

Expression

Plant

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1/Xpress beta4 1355T

Plasmid

#16040

Depositor

Filippo Giancotti

Publication

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Tags

6xHis and Xpress

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_Kaede

Plasmid

#62413

Purpose

MXS_chaining vector with Kaede

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Kaede

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_iRFP670

Plasmid

#62411

Purpose

MXS_chaining vector with iRFP670

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

iRFP670

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ265F2

Plasmid

#164722

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Publication

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)NG-UGI

Use

CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

Expression

Plant

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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asPink chromoprotein promoter-less

Plasmid

#118005

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, asPink

Use

Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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BC-A1-002

Plasmid

#78689

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Publication

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK

Plasmid

#62414

Purpose

MXS_chaining vector with PGK

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV

Plasmid

#62417

Purpose

MXS_chaining vector with CMV

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMX-hTAF10

Plasmid

#44340

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF10 (TAF10 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Magenta

Plasmid

#160446

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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