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pRS423-TyrSgH

Plasmid

#163973

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP713

Plasmid

#197208

Purpose

Expresses the protein of miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-dAsCas12f

Plasmid

#204641

Purpose

Expression of an inactive AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

inactive AsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D225A

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ MBIC5-mCer3

Plasmid

#74116

Purpose

For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

Depositor

Michael Tsang

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Transgenic construct

Tags

mCerulean 3

Promoter

Myl7 (Cmlc2)

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA3

Plasmid

#199729

Purpose

Golden Gate entry vector; 3rd gRNA under TaU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU3-sgRNA3 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA4

Plasmid

#199730

Purpose

Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C1-sgRNA4 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA5

Plasmid

#199731

Purpose

Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C3-sgRNA5 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA6

Plasmid

#199732

Purpose

Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.1-sgRNA6 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA7

Plasmid

#199733

Purpose

Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.2-sgRNA7 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA8

Plasmid

#199734

Purpose

Golden Gate entry vector; 8th gRNA under TaU6 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU6-sgRNA8 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP703

Plasmid

#197205

Purpose

Expresses the protein of emiRFP703 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKCZeta KR HA Tagged

Plasmid

#24608

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Mutation

K281R mutation in the ATP binding domain causing …

Available Since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Trip-GFP-hOCLN-ΔEL2

Plasmid

#32797

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of aa 200-238

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
Trip-GFP-hOCLN-ΔC

Plasmid

#32794

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 267-522

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pISceI-myl7:MBIC5-mCer3

Plasmid

#74117

Purpose

Zebrafish transgenic construct

Depositor

Michael Tsang

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Mrna generation for microinjection.

Tags

mCerulean 3

Expression

Mammalian

Promoter

SP6

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSB3K3-IC15

Plasmid

#165408

Purpose

Bacteria gene circuit on medium copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

IC15 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEBB-XIAP1-351

Plasmid

#11560

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 352-496

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pZmU3-gRNA

Plasmid

#53061

Purpose

Transcription of guide RNA (gRNA) in maize cells

Depositor

Caixia Gao

Article

Liang et al J Genet Genomics. 2014 Feb 20;41(2):63-8

Insert

zmU3 promoter - gRNA

Use

CRISPR; Plant expression

Promoter

maize U3

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab27 WT

Plasmid

#44832

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab27 (Rab27 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only