We narrowed to 27,060 results for: cas9 genes
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Plasmid#180471PurposeFor expression of Cas9 and gRNAs, multiple gRNAs cloning vectorDepositorTypeEmpty backboneUseCRISPRTagsCas9ExpressionPlantAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only
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pKM808
Plasmid#134181PurposeLentiviral sgRNA plasmid with blasticidin resistanceDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458 EQR
Plasmid#101731PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationEQR (D1135E, R1335Q, and T1337R mutations in Cas9)Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330 VRER
Plasmid#101729PurposeExpresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifsDepositorInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E, and T1337R mutation…Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p458 VRER
Plasmid#101728PurposeExpresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifsDepositorInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E, and T1337R mutation…Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p330 VQR
Plasmid#101730PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifsDepositorInsertSpCas9 VQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVQR (D1135V, R1335Q, and T1337R mutations in Cas9)Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR1003
Plasmid#71821PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A)DepositorInsertT7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCJJ339
Plasmid#117501PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
XX>sgRNA F+E
Plasmid#87543PurposeTemplate 2 (of 2) for U6>sgRNA cassette OSO-PCRDepositorTypeEmpty backboneAvailable SinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCJJ358
Plasmid#117505PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330 EQR
Plasmid#101733PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationEQR (D1135E, R1335Q, and T1337R mutations in Cas9)Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only