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pBF_HsPepT2

Plasmid

#73944

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT2 (SLC15A2 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pBF_HsPepT1

Plasmid

#73943

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT1 (SLC15A1 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn1_nLightG

Plasmid

#187179

Purpose

Expresses green norepinephrine indicator nLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightG

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p6347 MSCV-CMV-Flag-HA-Brd4-444-722

Plasmid

#31353

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 444-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-O2S

Plasmid

#21162

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4, Sox2 (SOX2, POU5F1 Human)

Use

Lentiviral; Reprogramming

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pGAPTrap TALEN 2

Plasmid

#83369

Purpose

Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN2

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAX198

Plasmid

#173042

Purpose

Custom vector for sgRNA library construction

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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