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We narrowed to 10,313 results for: otos

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Showing: 10041 - 10060 of 10313 results

  1. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. TFORF2120

    Plasmid
    #143871
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HOPX (HOPX Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143871-plasmid-map-sequence-id-338766

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  3. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  4. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  5. mCherry-CRY2-INPP5E(D556A)

    Plasmid
    #79563
    Purpose
    expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. *MtbCM-HiBiT-His

    Plasmid
    #228028
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Promoter
    T7 Promotor
    Available Since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    228028-plasmid-map-sequence-id-451757

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  12. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

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  13. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  20. pMRX-IP-EGFP-PA-BAX(mito, G108V)

    Plasmid
    #233343
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233343-plasmid-map-sequence-id-469412

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Showing: 10041 - 10060 of 10313 results