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pFA6a-mIAA7-mNeon-HISMX6

Plasmid

#247607

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

mNeonGreen

Expression

Yeast

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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EML6428_ pRL662-eGFP

Plasmid

#234350

Purpose

Expressed eGFP(S65T) in agrobacteria for tracing in planta

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Bacterial

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA7-1

Plasmid

#248530

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA7-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA7-3

Plasmid

#248531

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA7-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA8-2

Plasmid

#248532

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Publication

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA8-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGR721 - SLD3p-TP-DNAP1.Badboy2

Plasmid

#222013

Purpose

Expresses the Badboy2 TP-DNAP1 variant under control of a weak promoter for reducing p1 copy number

Depositor

Chang Liu

Publication

Rix et al Science. 2024 Nov 8;386(6722):eadm9073.

Insert

TP-DNAP1 BadBoy2

Expression

Yeast

Promoter

SLD3p

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2673

Plasmid

#241720

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSD marker for selection on blasticidin S

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_HA-VSVG

Plasmid

#212647

Purpose

piggyBac vector to express HA-VSVG

Depositor

Paul Blainey

Publication

Najia et al Cell. 2026 Sep 1:S0092-8674(26)00932-3.

Insert

VSVG

Use

Piggybac

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-SpyCatcher002-MBP

Plasmid

#216295

Purpose

Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher002-MBP

Tags

His6 tag and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV/eGFP-U6-sgRNA

Plasmid

#170544

Purpose

A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

Depositor

Jacob Giehm Mikkelsen

Publication

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE_hePOU_shp53

Plasmid

#206397

Purpose

Integration-free (episomal) expression of human ePOU and shRNA against p53.

Depositor

Ralf Jauch

Inserts

ePOU
shRNA against p53 (TP53 Human)

Expression

Mammalian

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

CYC1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

Plasmid

#173230

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Publication

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173231

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Publication

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry-Cbx5

Plasmid

#194547

Purpose

Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

Depositor

Denes Hnisz

Publication

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

Cbx9 (HP1a)

Tags

mCherry

Expression

Bacterial

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-moxBFP-IRES-Flpo

Plasmid

#194977

Purpose

AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Publication

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP, Flpo

Use

AAV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Publication

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Publication

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-HALO

Plasmid

#188943

Purpose

T7 expression vector for making his6- and HA3-tagged HALO protein

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-HALO

Expression

Bacterial

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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