We narrowed to 63,901 results for: cloning
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Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
ExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SESN2
Plasmid#34820DepositorAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MTPN
Plasmid#34829DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MPP6
Plasmid#34806DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PEPD
Plasmid#31328DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EFS-MCS-WPRE
Plasmid#163362PurposeThe empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectorsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNA500
Plasmid#149640Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.cspA_v1
Plasmid#133071PurposeEntry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseGateway CloningTags3C-His6 and His6-3CExpressionBacterialPromotercspAAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-NADAR
Plasmid#255737PurposeFor Gateway cloning of NADARDepositorAvailable sinceJuly 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDIV068
Plasmid#247961PurposeCloning vector containing A-PgpdA-PacI-Nt.BbvcI cassette-TtrpC-B sequences compatible with the DIVERSIFY gene landing platformDepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mEYFP
Plasmid#223955PurposeGateway cloning of genes of interest with an N-terminal V5-mEYFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEYFPAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH46 - MosTI [unc-119(partial) | MCS | gfp]
Plasmid#200366PurposeCloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.DepositorTypeEmpty backboneExpressionWormAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]
Plasmid#200365PurposeCloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.DepositorTypeEmpty backboneExpressionWormAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWX-R4DESTR2-PGK-Tk:Sh
Plasmid#114321PurposeLentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablationDepositorTypeEmpty backboneUseLentiviralAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE332
Plasmid#153241PurposeM1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by