We narrowed to 36,031 results for: CaS;
-
Plasmid#214032PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT-sm
Plasmid#214033PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bn R52A, R54A pMT2
Plasmid#206087PurposeCav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2DepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
An HA CAAX pMT2
Plasmid#206116Purposeexpression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tagDepositorAvailable SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bn CAAX pcDNA3
Plasmid#206088PurposeCav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motifDepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
An CAAX pMT2
Plasmid#206118Purposeexpression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motifDepositorAvailable SinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-CSK-P2A-mCherry BlastR
Plasmid#186742PurposeDox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR
Plasmid#186744PurposeEF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backboneDepositorInsertEF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP
UseLentiviralTagsP2A-BFPExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187611PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianPromoterEF1AAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187612PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain (R163A) with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationArg163AlaPromoterEF1AAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 TAPBPR-TM-TN6
Plasmid#178650PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)DepositorInsertTAPBPR (TAPBPL Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationE205K, R207E, Q209S, Q272S, switched transmembran…PromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine
Plasmid#178825PurposeNeuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tagDepositorInsertQuasAr6b_citrine
UseCre/Lox and LentiviralTagscitrineExpressionMammalianPromoterhSynAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K206A/K244A/L301R/V303R/V306R)
Plasmid#177138PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(K206A/K244A/L301R/V303R/V306R)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(L301R/V303R/V306R)
Plasmid#177137PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K206A/K244A/T304I)
Plasmid#177134PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only