We narrowed to 118,874 results for: mpi

1. pRNATU6.1 HDAC3 shRNA1

   Plasmid

   #112249

   Purpose

   HDAC3 KD

   Depositor

   Simone Di Giovanni

   Insert

   shRNA against mouse HDAC3 (Hdac3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

   Plasmid

   #159907

   Purpose

   Mutagenesis of Netrin1

   Depositor

   Larry Zweifel

   Insert

   Netrin1 (Ntn1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-rUSP19ER-siRNAresistant

   Plasmid

   #155251

   Purpose

   for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) siRNA resistant (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAMTA2.1.0-gDNA

   Plasmid

   #132456

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CAMTA2 (CAMTA2 Human)
   

   Use

   CRISPR

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-1-3xVSVG

   Plasmid

   #129568

   Purpose

   Empty backbone for VSVG tag

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1-3xGluGlu

   Plasmid

   #129571

   Purpose

   Empty backbone for GluGlu tag

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBD1.1.0-gDNA

   Plasmid

   #132444

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   MBD1 (MBD1 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. KSP_HNL

   Plasmid

   #129772

   Purpose

   Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

   Depositor

   William Hancock

   Insert

   KSP (kif11.L Frog)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Truncation at 367

   Available Since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Unc104_K560GFP

   Plasmid

   #129773

   Purpose

   Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

   Depositor

   William Hancock

   Insert

   Unc104 (unc-104 Nematode)
   

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 361, fused to kinesin-1 starting Al…

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p64T-XBMP2

    Plasmid

    #117253

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

    Insert

    BMP2
    

    Use

    Xenopus expression

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-XTbx5

    Plasmid

    #117247

    Purpose

    mRNA synthesis in situ hyb probes

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5 (tbx5-b Frog)
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSP64T-BMP4

    Plasmid

    #117254

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

    Insert

    BMP4
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-Pur-LPAR

    Plasmid

    #125205

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide

    Depositor

    Eric Davis

    Insert

    Lyn-Phospho-Activity-Reporter, Empty
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-mTRAF3ip3∆TM-CFP

    Plasmid

    #125182

    Purpose

    Expresses mouse TRAF3IP3 ∆TM in mammalian cells

    Depositor

    Shao-Cong Sun

    Insert

    TRAF3ip3 (Traf3ip3 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    K87R and S359P- See depositor comments below

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZSCAN18-donor

    Plasmid

    #113821

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP

    Depositor

    Kevin White

    Insert

    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    

    Use

    CRISPR

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7-5iX

    Plasmid

    #106991

    Purpose

    T7 transcription template for 5iX

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    5iX
    

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7-Xi3

    Plasmid

    #106992

    Purpose

    T7 transcription template for Xi3

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    Xi3
    

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAW5

    Plasmid

    #110221

    Purpose

    Cre-expression plasmid

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZNF561-donor

    Plasmid

    #113801

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF561 with GFP

    Depositor

    Kevin White

    Insert

    ZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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