We narrowed to 13,842 results for: CAR

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pLKO.1-TRC.mKO2_shB3GNT5.1

Plasmid

#110325

Purpose

TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

Plasmid

#128587

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

EF1α (1.1 kb short version)

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM5-C234S PHGDH

Plasmid

#83902

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Mutation

quikchanged cysteine 234 to serine

Promoter

CMV

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

Plasmid

#163176

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-4

Plasmid

#223225

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-3

Plasmid

#223224

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-1

Plasmid

#223223

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-835_NY-ESO-1_TCR_FAS/MyD88

Plasmid

#207498

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

FAS/MyD88, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH-C234S

Plasmid

#107724

Purpose

Expression of PHGDH C234S in bacteria

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

PHGDH (PHGDH Human)

Expression

Bacterial

Mutation

C234S

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pIG-834_NY-ESO-1_TCR_FAS/IL4R

Plasmid

#207497

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

FAS/IL4R, NY-ESO-1_TCR (FAS Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX

Plasmid

#124258

Purpose

Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmFbxw7_R505C

Plasmid

#160104

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

R505C

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

Plasmid

#153538

Purpose

AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

EF1α1.1

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-fd [Chronos-GFP]

Plasmid

#84482

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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GST-FUS 465-526

Plasmid

#44983

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (465-526aa) (FUS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 465-526

Promoter

tac

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_7

Plasmid

#60293

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC30A8 enhancer (SLC30A8 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSN879

Plasmid

#190693

Purpose

Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

Depositor

Shinsuke Niwa

Article

Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

Insert

mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)

Use

RNAi

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-STOP-TdT

Plasmid

#126425

Purpose

Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Mutation

alanine-6 of TdT has been changed to a STOP codon

Promoter

pCMV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

Plasmid

#169168

Purpose

Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6X Histidine Tag, TEV cleavage site, mCherry

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

Plasmid

#114094

Purpose

CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized AsCas12a-RR (S542R/K607R)

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

S542R and K607R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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