We narrowed to 11,561 results for: kars;

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pLX304 GDF11-V5 blast

Plasmid

#83098

Purpose

Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

GDF11 (GDF11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

Plasmid

#98975

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 4 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pUSE-RapR-Src(KD)-myc

Plasmid

#25934

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-Src (Fkbp1a Mouse)

Expression

Mammalian

Mutation

This pUSE-RapR-Src(KD)-myc plasmid contains the k…

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pLEXm-GST-ATG14

Plasmid

#99329

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

ATG14 (ATG14 Human)

Tags

GST-TEV tag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSC5GGv2

Plasmid

#188604

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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All-in-one split-ADAR2(E488Q, N496F)

Plasmid

#170148

Purpose

AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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LightR-iCre-miRFP670

Plasmid

#162158

Purpose

Expresses LightR-iCre-miRFP670 in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

LightR-iCre-miRFP670

Tags

NLS and miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM-KD

Plasmid

#25929

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Human, Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-KD plasmid contains the FA…

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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All-in-one split ADAR2(E488Q)

Plasmid

#170137

Purpose

AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-URA-hPOLISc

Plasmid

#60819

Purpose

to express human Pol iota recoded to yeast codons in yeast

Depositor

Youri Pavlov

Article

Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

Insert

POLI (POLI Human)

Tags

c-myc

Expression

Yeast

Mutation

optimized to yeast codon usage

Promoter

GAL1

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-dDrp1-StrepII

Plasmid

#174421

Purpose

Expresses D. melanogaster Drp1 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (Drp1 Fly)

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Human, Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-p38 (Mapk14 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066

Plasmid

#175587

Purpose

in vitro transcription of wt RBM8A 3'UTR

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A-3'UTR AluY-Sz WT

Use

In vitro transcription

Promoter

T7

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas8b2

Plasmid

#126488

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas7

Plasmid

#126489

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas5

Plasmid

#126490

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6

Plasmid

#126491

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (214-513aa)

Plasmid

#78095

Purpose

mammalian expression of GADD34 214-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 214-513

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
Venus-G protein Gamma 5

Plasmid

#42200

Depositor

Narasimhan Gautam

Article

Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

Insert

Gamma 5 (Gng5 Rat)

Tags

Venus(1-155)

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pEBG Jip3 (nterm)

Plasmid

#58942

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

GST

Expression

Mammalian

Mutation

deleted aa 271-1817

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-LmoCascade-IL1RNcrRNA

Plasmid

#126493

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCascade-HBE1crRNA

Plasmid

#126494

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo HBE1 cr06

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

PH-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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