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Showing: 2501 - 2550 of 11683 results

  4. pBCSK_HIS3_I

    Plasmid
    #109058
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109058-plasmid-map-sequence-id-209548

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  9. pTet_KaeCanABC(G104K,G189K)

    Plasmid
    #248958
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(G104K,G189K)
    Expression
    Bacterial
    Mutation
    G104K,G189K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248958-plasmid-map-sequence-id-500779

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  10. pTet_PflHamMA(-cJun)B

    Plasmid
    #248940
    Purpose
    Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker
    Depositor
    Jennifer Doudna
    Insert
    flHamMA(-cJun)B
    Expression
    Bacterial
    Mutation
    c-Jun insertion, HamA domain deletion
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248940-plasmid-map-sequence-id-500774

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  11. pLG017_FLAG-Avs1a(I134K,I234K)

    Plasmid
    #248949
    Purpose
    AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K)
    Depositor
    Jennifer Doudna
    Insert
    FLAG-Avs1a(I134K,I234K)
    Tags
    3xFLAG
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248949-plasmid-map-sequence-id-500770

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  12. pTet_PflHamM(I118K,I218K)AB

    Plasmid
    #248937
    Purpose
    Hachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K)
    Depositor
    Jennifer Doudna
    Insert
    PflHamM(I118K,I218K)AB
    Expression
    Bacterial
    Mutation
    I118K,I218K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248937-plasmid-map-sequence-id-500765

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  13. pDest_PANCEd_AraC_R2_S170_LOV_P2.1_C3

    Plasmid
    #242155
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P2.1.3 variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV P2.1.3
    Expression
    Bacterial
    Mutation
    AraC: E3K, E144K, R146L, A152T, S225R, R251L; AsL…
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242155-plasmid-map-sequence-id-492368

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  14. pDest_PANCEd_AraC_R2_LDelK

    Plasmid
    #242156
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE/RAMPhaGE Linker Library variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV LDelK
    Expression
    Bacterial
    Mutation
    AraC: E3K, R146L, A152T, R251L; Left Linker: GS -…
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    242156-plasmid-map-sequence-id-486033

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  26. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid
    #160588
    Purpose
    Nickase D10A Cas9 for Nt fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160588-plasmid-map-sequence-id-322078

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  28. pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo

    Plasmid
    #128463
    Purpose
    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128463-plasmid-map-sequence-id-270529

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  29. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  30. pCASCADE-udhA-zwf

    Plasmid
    #87153
    Purpose
    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87153-plasmid-map-sequence-id-174933

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  31. pJK653

    Plasmid
    #71630
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine, phenylalanine-79 …
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71630-plasmid-map-sequence-id-131649

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  32. pJK654

    Plasmid
    #71631
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine, phenylalanine-79 to…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71631-plasmid-map-sequence-id-131651

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  33. pJK655

    Plasmid
    #71632
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate, phenylalanine-79 …
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71632-plasmid-map-sequence-id-131652

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  34. pJK656

    Plasmid
    #71633
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine, phenylalanine-79 to …
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71633-plasmid-map-sequence-id-131653

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  35. pJK659

    Plasmid
    #71634
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine, phenylalanine-79 t…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71634-plasmid-map-sequence-id-131654

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  36. pJK660

    Plasmid
    #71635
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine, phenylalanine-79…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71635-plasmid-map-sequence-id-131655

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  37. pJK661

    Plasmid
    #71636
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg/Phe79>Trp mutant (TdPurE2-K41R,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine, phenylalanine-79 t…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71636-plasmid-map-sequence-id-131656

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  38. pJK662

    Plasmid
    #71637
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan, cysteine-…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71637-plasmid-map-sequence-id-131657

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  39. pJK689

    Plasmid
    #71642
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine, phenylalanine-79…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71642-plasmid-map-sequence-id-131663

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  40. pJK657

    Plasmid
    #71647
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-40 to alanine, phenylalanine-79…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71647-plasmid-map-sequence-id-131642

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  41. pJK658

    Plasmid
    #71648
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-40 to asparagine, phenylalanine…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71648-plasmid-map-sequence-id-131644

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  42. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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  43. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

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  44. pHL 1915

    Plasmid
    #53022
    Purpose
    AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)
    Depositor
    Han Lim
    Insert
    GFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    pLlacO-1 and fimS fusion
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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Showing: 2501 - 2550 of 11683 results