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Showing: 1001 - 1020 of 12329 results

  2. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

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  10. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #179682
    Purpose
    Expression of base editor in mammalian cells and for cell sorting
    Depositor
    David Liu
    Article
    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387
    Insert
    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S1330I A1341V N1342S E1370K T1380I
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179682-plasmid-map-sequence-id-355098

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  14. epi-BE4max

    Plasmid
    #135975
    Purpose
    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135975-plasmid-map-sequence-id-292615

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  16. pDL17

    Plasmid
    #106483
    Purpose
    pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    exo
    lacI
    rhaR
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106483-plasmid-map-sequence-id-334156

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  19. epi-ABEmax

    Plasmid
    #135974
    Purpose
    Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1020 of 12329 results