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Plasmid#91914PurposeExpresses CgCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertCgCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_EWSR1_His6
Plasmid#26364DepositorInsertEWSR1 (EWSR1 Human)
UseEntry vectorTagsFLAG/HA and His6ExpressionMutationdelta 657 (stop codon)PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSAH0016
Plasmid#79824PurposeDual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicolDepositorInsertscat
aadA
UseTagsExpressionMutationPromoterAATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST_EWSR1_G2A_R650H
Plasmid#26383DepositorInsertEWSR1 (EWSR1 Human)
UseTagsFLAG/HAExpressionMammalianMutationR650HPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_EWSR1_G2A_R650H
Plasmid#26372DepositorInsertEWSR1 (EWSR1 Human)
UseEntry vectorTagsExpressionMutationR650HPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant
Plasmid#106112PurposeInducible gene expression of FlagHA-PRKRA sgRNA PAM mutantDepositorInsertPRKRA (PRKRA Human)
UseTagsFlag and HAExpressionMammalianMutationsilent mutation of Gly32 (GGG-->GGT)PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK c2orf42 3'UTR
Plasmid#19862DepositorInsertc2orf42 3'UTR (C2orf42 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK ADRB2 3'UTR
Plasmid#19867DepositorInsertADRB2 3'UTR (ADRB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545AK570A
Plasmid#14105DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Gln545 to Ala and Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847
Plasmid#91915PurposeExpresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertCg2Cas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST_EWSR1_C2G_R650G
Plasmid#26382DepositorInsertEWSR1 (EWSR1 Human)
UseTagsFLAG/HAExpressionMammalianMutationR650GPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K570A
Plasmid#14102DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK GSC 3'UTR
Plasmid#19859DepositorInsertGSC 3'UTR (GSC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK RBJ 3'UTR
Plasmid#19866DepositorInsertRBJ 3'UTR (DNAJC27 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533A
Plasmid#14100DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545A
Plasmid#14101DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Gln545 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AQ545A
Plasmid#14103DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Gln545 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only