Skip to main content
Addgene

We narrowed to 4,566 results for: POR C

Showing: 1001 - 1020 of 4566 results
  1. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid
    #182347
    Purpose
    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

    Plasmid
    #182349
    Purpose
    mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Insert
    his-58, eGFP, mCherry
    Use
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pDEST-pSP172BSSPE-Swa1::HA-RfA

    Plasmid
    #186404
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pDEST-pSP172BSSPE-pFOGc::venus-RfA

    Plasmid
    #186400
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pDEST-pSP172BSSPE-Swa1::venus-RfA

    Plasmid
    #186398
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1020 of 4566 results