We narrowed to 2,475 results for: T2A

1. pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

   Plasmid

   #225586

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   F8.2(S54L+T127A)-T2A-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV pSYN eGFP T2A SP HALO HAPLN1

   Plasmid

   #228199

   Purpose

   AAV vector expressing GFP and HaloTag-HAPLN1 under synaptophysin promoter

   Depositor

   Sungjin Park

   Article

   Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

   Insert

   HAPLN1 (Hapln1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human synaptophysin promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-hU6-cs1-PuroR-T2A-BFP

   Plasmid

   #230934

   Purpose

   gRNA cloning vector with direct capture sequence 1 in stem loop 2

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

   Plasmid

   #226834

   Purpose

   Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   Cas9-t2A-GFP
   4xU6:sgRNA
   

   Use

   CRISPR

   Promoter

   Olactb and U6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE3G-FLAG-human cGAS N-T2A-deltaN-HA

   Plasmid

   #186886

   Purpose

   Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Human cGAS with T2A ribosome skipping sequences between N-terminus (1-159 a.a.) and C-terminus (160-522 a.a.) (Adamts1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Mutation

   Insertion of T2A ribosome skipping site

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

   Plasmid

   #194230

   Purpose

   Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-LCB3-SynNotch-T2A-mCherry
   

   Use

   Lentiviral

   Mutation

   E45D mutation in LCB3

   Promoter

   PGK

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

   Plasmid

   #194232

   Purpose

   Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   EF1a-LCB3-PDGFR TMD-T2A-mCherry
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

   Plasmid

   #194234

   Purpose

   Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

   Plasmid

   #223504

   Purpose

   Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

   Depositor

   James Frank

   Insert

   dead-mutant SNAP-tag and EGFP
   

   Expression

   Mammalian

   Mutation

   SNAP-tag C145A

   Promoter

   hSyn

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX-Sox17-p2A-Erg-t2A-GFP

    Plasmid

    #222496

    Purpose

    mouse fibroblast-to-endothelial cell direct reprogramming

    Depositor

    Li Qian

    Article

    Farber et al Nat Commun. 2024 May 16;15(1):4170. doi:

    Inserts

    Sox17 (Sox17 Mouse)
    Erg (Erg )
    EGFP
    

    Use

    Retroviral

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Puro cTnT CCND2-T2A-ChloC

    Plasmid

    #179841

    Purpose

    donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    CRISPR and TALEN; Donor plasmid

    Tags

    chloride-conducting ChR (ChloC)

    Expression

    Mammalian

    Promoter

    Troponin T promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-Puro cTnT CCND2-T2A-iChloC

    Plasmid

    #179842

    Purpose

    donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    CRISPR and TALEN; Donor plasmid

    Tags

    improved chloride-conducting ChR (iChloC)

    Expression

    Mammalian

    Promoter

    Troponin T promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Puro cTnT CCND2-T2A-luciferase

    Plasmid

    #179844

    Purpose

    donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    CRISPR, Luciferase, and TALEN ; Donor plasmid

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Troponin T promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)

    Plasmid

    #221330

    Purpose

    Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-IRSp53 and HA-Rac1(G12V) (Baiap2, Rac1 Mouse)
    

    Use

    Retroviral

    Mutation

    G12V

    Promoter

    TRE3G

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p186-RV-CAG-dio-mScarlet-T2A-mVenus

    Plasmid

    #204740

    Purpose

    Conditional expression of target gene mVenus with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    mVenus
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

    Plasmid

    #195501

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4

    Plasmid

    #193295

    Purpose

    episomal vector expressing mCherry + human SOX2A61V + KLF4

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2AV-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
    

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid

    #141100

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJET-63-SFFV-T2A-BlaR-polyA-R11a

    Plasmid

    #179894

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Expression

    Bacterial and Mammalian

    Promoter

    SFFV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJET-61-SFFV-T2A-PuroR-polyA-R11a

    Plasmid

    #179892

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Expression

    Bacterial and Mammalian

    Promoter

    SFFV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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