We narrowed to 3,071 results for: bad

1. pCK314

   Plasmid

   #110545

   Purpose

   Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   Modified PrhaBAD (CRP-binding sites removed)
   yfp
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-shNCLX-2

   Plasmid

   #181871

   Purpose

   Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   solute carrier 8 family member B1 (Slc8b1 Mouse)
   

   Use

   AAV, Adenoviral, and Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-shNCLX-1

   Plasmid

   #181870

   Purpose

   Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   solute carrier 8 family member B1 (Slc8b1 Mouse)
   

   Use

   AAV, Adenoviral, and Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcdna DSG-2 TS

   Plasmid

   #89465

   Purpose

   DSG-2 tension sensor, using TSmod inserted in human DSG-2

   Depositor

   Daniel Conway

   Article

   Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

   Insert

   desmoglein-2 (DSG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCapV

   Plasmid

   #183028

   Purpose

   the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

   Depositor

   Ute Romling

   Article

   Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

   Insert

   patatin-like phospholipase CapV
   

   Use

   Tags

   No

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_GNAT1_G-alpha

   Plasmid

   #109946

   Purpose

   Protein expression and purification of GNAT1_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAT1_G-alpha (GNAT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBICI_high ori

   Plasmid

   #179273

   Purpose

   Expresses CuCBP and BaSP in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6-TEV-spTorA-GFP(C)

   Plasmid

   #169041

   Purpose

   GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   H6-spTorA-GFP
   

   Use

   Tags

   6xHis, TEV cleavage site, and spTorA (signal pept…

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCapVQ329R

   Plasmid

   #183029

   Purpose

   the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28

   Depositor

   Ute Romling

   Article

   Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

   Insert

   patatin-like phospholipase variant CapVQ329R
   

   Use

   Tags

   No

   Expression

   Bacterial

   Mutation

   glutamine 329 changed to arginine

   Promoter

   pBAD promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-shMcu-1

    Plasmid

    #181868

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MCU-EGFP

    Plasmid

    #181866

    Purpose

    Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV and Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-shMcu-2

    Plasmid

    #181867

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EGFP.T2A.NCLX

    Plasmid

    #181873

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    

    Use

    AAV and Adenoviral

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    synthetic hybrid CAG promoter and synthetic hybri…

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEL1200

    Plasmid

    #138526

    Purpose

    For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

    Insert

    mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)
    

    Use

    Tags

    N-terminal His tag and TEV cleavage site on MlaD

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-MCU-tDimer

    Plasmid

    #181865

    Purpose

    Expresses tDimer-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV and Adenoviral

    Tags

    tDimer

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid

    #241026

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven empty sgRNA
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. COVID-SARS2 NSP14/10

    Plasmid

    #159613

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

    Depositor

    Opher Gileadi

    Article

    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

    Inserts

    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6, TEV cleavage site and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid

    #181872

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    

    Use

    AAV and Adenoviral

    Tags

    HA, T2A, and myc

    Expression

    Mammalian

    Mutation

    includes a large portion of the Slc8b1 3'UTR…

    Promoter

    synthetic hybrid CAG promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CIDAR MoClo Parts Kit

    Plasmid Kit

    #1000000059

    Purpose

    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli

    Depositor

    Douglas Densmore

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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