We narrowed to 6,716 results for: human c myc
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Plasmid#208623Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)DepositorInserthuman RPL3 (ENST00000216146) (RPL3 Human)
Tags6xHis and Twin StrepExpressionBacterialPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PTEN-shRNA-1320
Plasmid#25638DepositorAvailable SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PTEN-shRNA-3001
Plasmid#25639DepositorAvailable SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo
Plasmid#191397PurposesgRNA targeting alpha-satellites on human chromosome 9DepositorInsertsgRNA targeting alpha satellites on human chromosome 9
UseLentiviralExpressionMammalianPromoterU6Available SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO2_F
Plasmid#146315PurposeMammalian Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
ExpressionMammalianMutationone silent mutation C840T and sequence differs in…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3L-TwStrep
Plasmid#208649Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949DepositorInserthuman RPL3L (ENST00000268661) (RPL3L Human)
Tags6xHis and Twin StrepExpressionBacterialMutationcontain rs753338989, rs746044959, rs752134949, pr…PromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpG
Plasmid#179081PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variantDepositorInsertSpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationD1135L, S1136W, G1218K, E1219Q, R1335Q, T1337RPromoterCAGAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-Cx32D178Y-IRES-GCaMP6s
Plasmid#216796PurposeInducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6sDepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpRY
Plasmid#179082PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variantDepositorInsertSpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…PromoterCAGAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-EGFP-GPI-stop-L2
Plasmid#186355PurposeEntry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequencesDepositorInsertsignal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
UseExpression of a fluorescent membrane markerTagsEGFPAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Mcl-1 mut
Plasmid#19133DepositorInsertMcl-1 promoter (MCL1 Human)
UseLuciferaseExpressionMammalianMutationNFkB binding-site mutant, the sequence GGGGTCTTCC…Available SinceOct. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
R777-E101 Hs.HRAS
Plasmid#70385PurposeGateway ORF clone of human HRAS [NM_005343.2] with stop codon (for native or N-terminal fusions)DepositorInsertHRAS (HRAS Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas12aUltra_5XNLS
Plasmid#218775PurposeHis-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLSDepositorInsert5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
UseCRISPRTags6XHis (C terminal), HA, Nucleoplasmin, SV40, and …ExpressionBacterialMutationNucleotides mutations were made to encode for arg…Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-SPIC
Plasmid#162825PurposeDoxycycline-inducible overexpression of human SPICDepositorAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 no-stop
Plasmid#123207PurposeGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneMutationStop codon removedAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianPromoterpTRE bidirectional tet-onAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GCN5 FL-dCas9-CBP core
Plasmid#179556Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GCN5 FL-dCas9-p300 core
Plasmid#179554Purposeencodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ngn3ER-PuroR-mCherry
Plasmid#197385PurposeTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)DepositorInsertNgn3ER-2A-PuroR-2A-mCherry
UseLentiviralExpressionMammalianPromoterEF1AAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only