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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pK3F-GAL1

Plasmid

#85772

Purpose

N-ICE plasmid pK3F-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS-GAL1

Plasmid

#85778

Purpose

N-ICE plasmid pK3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-POL30-3'

Plasmid

#99546

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC25SUP35

Plasmid

#1096

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
YIp211-P30-His-pol30(K164R)-3'

Plasmid

#99548

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pMM2_4

Plasmid

#183057

Purpose

HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mRuby2
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mRuby2 and mTurquoise2

Expression

Yeast

Promoter

HAA1 and YGP1

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM0623

Plasmid

#128998

Purpose

pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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YIp204-P30-His-POL30

Plasmid

#99550

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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