We narrowed to 7,002 results for: tac
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HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
TagsHis and MBPExpressionBacterialPromotertacAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-rhodopsin-CTS
Plasmid#86071PurposeA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.DepositorInsertRhodopsin (RHO Bovine)
TagsGSTExpressionBacterialMutationcontains the region from 309-348 of RhodopsinPromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin W403A
Plasmid#45974DepositorInsertParkin W403A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin C431S
Plasmid#45976DepositorInsertParkin C431S (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhB D101N
Plasmid#108695PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutationDepositorInsertrnhB
TagsGSTExpressionBacterialMutationD101N catalytic site mutationPromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Snf1-cat
Plasmid#52683Purposeexpression of the Snf1 kinase domain in bacteriaDepositorInsertSNF1 (SNF1 Budding Yeast)
TagsGSTExpressionBacterialMutationencodes kinase domain only, aa 41-315PromotertacAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin F463A
Plasmid#45975DepositorInsertParkin F463A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlluciferase
Plasmid#63853PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 L182A/D183A/I184A/G185A
Plasmid#67608Purposebacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
TagsGSTExpressionBacterialMutationcatalytic mutant LDIG(182-185)AAAAPromotertacAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-Mst1 SARAH
Plasmid#84297PurposeExpresses Mst1 SARAH domain for interaction studies.DepositorAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin F146A
Plasmid#45973DepositorInsertParkin F146A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_T234A_S235A
Plasmid#40634DepositorInsertPOU5F1 (POU5F1 Human)
TagsGST, TEV cleavage, and Thrombin cleavageExpressionMammalianMutationT234A_S235APromotertacAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialPromotertacAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX_K1033R
Plasmid#221378PurposeExpresses ATRX_K1033R in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
ExpressionBacterialMutationChanged lysine 1033 to argininePromotertacAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM_K819R
Plasmid#221380PurposeExpresses FANCM_K819R in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
ExpressionBacterialMutationChanged lysine 819 to argininePromotertacAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only
