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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-SypGFP

Plasmid

#172380

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + SypEGFP

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-mito-eqFP650

Plasmid

#174179

Purpose

Express eqFP650 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

eqFP650 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-DsRed-Express2

Plasmid

#111257

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

PpsbA

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-jGCaMP8s

Plasmid

#172389

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

GCaMP8s

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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AA294

Plasmid

#215968

Purpose

Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U1a_v1

Use

Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA332

Plasmid

#216053

Purpose

Fragmid fragment: (C' terminus) 3x NLS sequences

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35-mut

Plasmid

#87341

Purpose

To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDAS12342_PEAR-GFP_2in1

Plasmid

#177184

Purpose

PEAR-GFP plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mAmetrine1.1-WPRE

Plasmid

#193340

Purpose

AAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLicC-MBP-APETx2

Plasmid

#72668

Purpose

Periplasmic expression of APETx2 in E.coli

Depositor

Glenn King

Article

Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

Insert

APETx2

Tags

His tag, MBP, MalE signal sequence for periplasmi…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFX-mKate2-Rab11

Plasmid

#174463

Purpose

Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mKate2 with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3

Plasmid

#191752

Purpose

Expression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-Ef1a-TVA-2A-oG

Plasmid

#172359

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-oG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

Plasmid

#45579

Purpose

Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani K227M

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-CALPAIN-7-mCherry

Plasmid

#180652

Purpose

Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GCACCCAUACCUUUACAUU

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

Myc and PDGFR

Expression

Mammalian

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-Integrin-Alpha2-N-18

Plasmid

#57389

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMX-mTAF6

Plasmid

#44337

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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