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pNIC28-BpWcbI

Plasmid

#125591

Purpose

Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

Insert

BpWcbI (wcbI Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pClodAcytCh-GFP-P4M

Plasmid

#113346

Purpose

Bacterial expression of Cherry and GFP-SidM-P4M domain (2 copies)

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

Cherry
GFP-SidM-P4M domain (2 copies of P4M in tandem)

Expression

Bacterial

Promoter

ProD and proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBHDuet50K

Plasmid

#118399

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Expression

Bacterial

Mutation

incorporation of oligonucleotide during cloning

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL010313 SPR

Plasmid

#115385

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

G-protein coupled receptor daf-37

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL007405 SP R

Plasmid

#115393

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL007405 SP R (SPR Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL012796

Plasmid

#115383

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL012796 (LOC5576812 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL007169 AstA R

Plasmid

#115400

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL007169 AstA R (AaeL_AAEL007169 )

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL006283 MS R

Plasmid

#115386

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL006283 MS R (LOC5567691 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) short BP-3'SS

Plasmid

#111333

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

shorter branch to 3' splice site distance

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3MCSpA

Plasmid

#109485

Purpose

Drive inserted gene in osteoblasts.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049d

Plasmid

#96862

Purpose

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049e

Plasmid

#96863

Purpose

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNfs1

Plasmid

#102967

Purpose

Suppression of mNfs1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNfs1

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-aVent1-pgk-hph

Plasmid

#74916

Purpose

Mammalian expresion of aVent1

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Vent1

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-aBsx-pgk-hph

Plasmid

#74913

Purpose

Mammalian expression of aBsx

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Bsx

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ti2

Plasmid

#59631

Purpose

Expression of shRNA against human T

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

T

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-Ti1

Plasmid

#59630

Purpose

Expression of shRNA against human T

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

T

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pME18ST-k5310

Plasmid

#52388

Purpose

Aedes aegypti NPY-like receptor AAEL005310 (NPYLR4) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR4

Expression

Mammalian

Mutation

*

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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