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pGV13 (CK2alpha K68M, CK2beta)

Plasmid

#27094

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha K68M (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA on CK2alpha and Myc on CK2beta

Expression

Mammalian

Mutation

K68M on CK2alpha

Promoter

bidirectional tet-responsive promoter and bidirec…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#1-puro

Plasmid

#231977

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

Plasmid

#221385

Purpose

Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

Depositor

Junwei Shi

Article

Ngiow et al Cell. 2024 Aug 8;187(16):4336-4354.e19.

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJV137

Plasmid

#124256

Purpose

Lentiviral vector (pRRL) containing Her2-ITD specific T cell receptor

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (ERBB2 Human)

Use

Lentiviral

Promoter

MSCV

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-GNL

Plasmid

#188232

Purpose

Polycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vector

Depositor

Young Tang

Article

Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

Insert

Glis1, NANOG and LIN28A separated by 2A sequence (GLIS1, NANOG, LIN28A Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2

Plasmid

#173952

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pICOZ-SB100X[EF1α-CD19 4-1BB]

Plasmid

#218328

Purpose

Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A3-P2A-mCherry

Plasmid

#203863

Purpose

Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A3 (NR4A3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.L276V.K312A

Plasmid

#238945

Purpose

Green glucose sensor, moderate sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3A-Fc(DAPA)-AviTag-6xHis

Plasmid

#156972

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3A (SEMA3A Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt[EF1α-copGFP]

Plasmid

#218325

Purpose

Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tc1-1_Xt

Plasmid

#217136

Purpose

Mammalian expression vector for expression of Tc1-1_Xt transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Tc1-1_Xt transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tc1-2_ST

Plasmid

#217232

Purpose

Mammalian expression vector for expression of Tc1-2_ST transposase (CMV promoter)

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Tc1-2_ST transposase

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST

Plasmid

#217289

Purpose

Donor plasmid of Tc1-2_ST Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-2_ST transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-piggyBac-1_AMi

Plasmid

#217236

Purpose

Donor plasmid of piggyBac-1_AMi Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of piggyBac-1_AMi transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-tdTHC

Plasmid

#112621

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-tdTomato (H2BC3 Human, Anthozoa)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Mcl-1-pLVX

Plasmid

#166752

Purpose

Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Mcl-1 (MCL1 Human)

Use

Lentiviral

Tags

mCerulean3

Promoter

CMV

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ROBO4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156864

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO4 (ROBO4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)

Plasmid

#198058

Purpose

Generation of rodent iPS cells from somatic cells

Depositor

Hiromitsu Nakauchi

Article

Yamaguchi et al Methods Mol Biol. 2014;1210:143-50. doi:

Insert

mOct4, mKlf4, mSox2 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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